EPAS1 抗体 (C-Term)
Quick Overview for EPAS1 抗体 (C-Term) (ABIN7602827)
抗原
See all EPAS1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- C-Term
-
原理
- Anti-HIF-2-alpha/EPAS1 Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-HIF-2-alpha/EPAS1 Antibody Picoband® (ABIN7602827). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human HIF-2-alpha/EPAS1, which shares 94.4% amino acid (aa) sequence identity with both mouse and rat HIF-2-alpha/EPAS1.
-
亚型
- IgG
-
-
anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 339-541) antibody
EPAS1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 21-336) antibodyEPAS1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Endothelial PAS Domain Protein 1 (EPAS1) (Ser343) antibodyEPAS1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 680-870) antibodyEPAS1 适用: 人 IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1H3E12 unconjugated
anti-Endothelial PAS Domain Protein 1 (EPAS1) (C-Term) antibodyEPAS1 适用: 人, 大鼠, 小鼠, 鱼 WB, IHC, ELISA, IP, ICC, FACS, IF, ChIP, GS 宿主: 兔 Polyclonal unconjugated
anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 28-347) antibodyEPAS1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1G2 unconjugated
anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 588-870) antibodyEPAS1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Endothelial PAS Domain Protein 1 (EPAS1) antibodyEPAS1 适用: 人 WB, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 535-631) antibodyEPAS1 适用: 人, 大鼠, 小鼠, Hamster WB, IHC, ELISA, IP, ICC, FACS, IF, ChIP 宿主: 小鼠 Monoclonal ep190b unconjugated
anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 1-130) antibodyEPAS1 适用: 人, 大鼠, 小鼠, 猴, 绵羊 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Tian, H., McKnight, S. L., Russell, D. W. : Endothelial PAS domain protein 1 (EPAS1), a transcription factor selectively expressed in endothelial cells. Genes Dev. 11: 72-82, 1997. 2. Sood, R., Zehnder, J. L., Druzin, M. L., Brown, P. O. : Gene expression patterns in human placenta. Proc. Nat. Acad. Sci. 103: 5478-5483, 2006. 3. Henderson, J., Withford-Cave, J. M., Duffy, D. L., Cole, S. J., Sawyer, N. A., Gulbin, J. P., Hahn, A., Trent, R. J., Yu, B. : The EPAS1 gene influences the aerobic-anaerobic contribution in elite endurance athletes. Hum. Genet. 118: 416-423, 2005. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))
-
别名
- EPAS1
-
背景
-
Synonyms: Cyclin-dependent kinase inhibitor 1C, Cyclin-dependent kinase inhibitor p57, p57Kip2, CDKN1C, KIP2
Tissue Specificity: Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. Expressed in the eye. High levels are seen in the placenta while low levels are seen in the liver.
Background: HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16. The predicted mouse protein is 88 % identical to human EPAS1. The human EPAS1 gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of EPAS1 and AHR are similar, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event. Moreover, the predicted protein shares 48 % sequence identity with HIF1-alpha, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia. Like HIF1A, EPAS1 binds to and activates transcription from the HIF1A response element derived from the 3-prime flanking region of the EPO gene. EPAS1 is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo. Furthermore, EPAS1 may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia. HIF2A is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined. In addition, The variation in EPAS1 influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration.
-
分子量
- 96 kDa
-
基因ID
- 2034
-
UniProt
- Q99814
-
途径
- Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect
抗原
-
