Actin 抗体 (C-Term)
Quick Overview for Actin 抗体 (C-Term) (ABIN7602771)
抗原
See all Actin (ACTA1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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原理
- Anti-Actin/ACTA1 Antibody Picoband® (monoclonal, 3H5)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Actin/ACTA1 Antibody Picoband® (monoclonal, 3H5) (ABIN7602771). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human Actin, identical to the related mouse and rat sequences.
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亚型
- IgG1
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anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 277-308), (C-Term) antibody
ACTA1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibodyACTA1 适用: 人, 大鼠, 斑马鱼, 兔, Pig, 小鸡, 猴 WB, ELISA, IHC, IHC (p), IHC (fro), IEM 宿主: 小鼠 Monoclonal HHF35 unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 346-375) antibodyACTA1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 3-377) antibodyACTA1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibodyACTA1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Monoclonal 25E3 unconjugated Recombinant Antibody
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 1-129) antibodyACTA1 适用: 人, 斑马鱼, 鱼 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 346-375), (C-Term) antibodyACTA1 适用: 人 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB35391 unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibodyACTA1 适用: 人 WB, IHC (p), FACS 宿主: 小鼠 Monoclonal 7C1 unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 7-377) antibodyACTA1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibodyACTA1 适用: 小鸡 WB, IHC, IF 宿主: 小鼠 Monoclonal C4 unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
1. Gunning,P., Ponte,P., Okayama,H., Engel,J., Blau,H. and Kedes,L.Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.Mol. Cell. Biol.1983, 3 (5), 787-795. 2. Goebel,H.H., Brockmann,K., Bonnemann,C.G., Warlo,I.A., Hanefeld,F.,Labeit,S., Durling,H.J. and Laing,N.G. Actin- related myopathy without any missense mutation in the ACTA1 Gene.J. Child Neurol.2004, 19 (2), 149-153. 3. Laing,N.G., Clarke,N.F., Dye,D.E., Liyanage,K., Walker,K.R.,Kobayashi,Y., Shimakawa,S., Hagiwara,T., Ouvrier,R., Sparrow,J.C., Nishino,I., North,K.N. and Nonaka,I.Actin mutations are one cause of congenital fibre type disproportion.Ann. Neurol.2004, 56 (5), 689-694. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))
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别名
- ACTA1
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背景
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Synonyms: Desmin, DES
Background: Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90 % sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.
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分子量
- 43 kDa
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基因ID
- 58
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UniProt
- P68133
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途径
- Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
抗原
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