PDK3 抗体 (AA 84-406)
Quick Overview for PDK3 抗体 (AA 84-406) (ABIN7602555)
抗原
See all PDK3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 84-406
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原理
- Anti-PDK3 Antibody Picoband®
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交叉反应 （详细）
- No cross reactivity with other proteins.
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产品特性
- Anti-PDK3 Antibody Picoband® (ABIN7602555). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PDK3 recombinant protein (Position: Y84-Q406). Human PDK3 shares 97.2% amino acid (aa) sequence identity with mouse PDK3.
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亚型
- IgG
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anti-Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) (AA 174-263) antibody
PDK3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2B11 unconjugated
anti-Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) antibodyPDK3 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) (AA 174-263) antibodyPDK3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A1 unconjugated
anti-Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) (AA 137-406) antibodyPDK3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) (Center) antibodyPDK3 适用: 人 ICC, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) antibodyPDK3 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB23090 unconjugated
anti-Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) (AA 1-30), (N-Term) antibodyPDK3 适用: 人 WB 宿主: 兔 Polyclonal RB3039 unconjugated
anti-Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) (N-Term) antibodyPDK3 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 斑马鱼, 犬, 马, 兔, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) (AA 131-362) antibodyPDK3 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) antibodyPDK3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Baker, J. C., Yan, X., Peng, T., Kasten, S., Roche, T. E. Marked differences between two isoforms of human pyruvate dehydrogenase kinase. J. Biol. Chem. 275: 15773-15781, 2000. 2. Degenhardt, T., Saramaki, A., Malinen, M., Rieck, M., Vaisanen, S., Huotari, A., Herzig, K.-H., Muller, R., Carlberg, C. Three members of the human pyruvate dehydrogenase kinase gene family are targets of the peroxisome proliferator-activated receptor beta/delta. J. Molec. Biol. 372: 341-355, 2007. 3. Gudi, R., Bowker-Kinley, M. M., Kedishvili, N. Y., Zhao, Y., Popov, K. M. Diversity of the pyruvate dehydrogenase kinase gene family in humans. J. Biol. Chem. 270: 28989-28994, 1995. Note: Erratum: J. Biol. Chem. 271: 1250 only, 1996. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- PDK3 (Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3))
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别名
- PDK3
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背景
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Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70 kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
Tissue Specificity: Expressed in all tissues.
Background: Pyruvate dehydrogenase lipoamide kinase isozyme 3, mitochondrial is an enzyme that in humans is encoded by the PDK3 gene. The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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分子量
- 47 kDa
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基因ID
- 5165
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UniProt
- Q15120
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途径
- PI3K-Akt Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect
抗原
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