CARD10 抗体 (AA 63-1014)
Quick Overview for CARD10 抗体 (AA 63-1014) (ABIN7602201)
抗原
See all CARD10 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 63-1014
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原理
- Anti-CARD10 Antibody Picoband®
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交叉反应 (详细)
- No cross-reactivity with other proteins
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产品特性
- Anti-CARD10 Antibody Picoband® (ABIN7602201). Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human CARD10 recombinant protein (Position: E63-R1014). Human CARD10 shares 90.7% amino acid (aa) sequence identity with mouse CARD10.
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亚型
- IgG
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL
1. Gaide, O., Martinon, F., Micheau, O., Bonnet, D., Thome, M., Tschopp, J. Carma1, a CARD-containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF-kappa-B activation. FEBS Lett. 496: 121-127, 2001. 2. Grabiner, B. C., Blonska, M., Lin, P.-C., You, Y., Wang, D., Sun, J., Darnay, B. G., Dong, C., Lin, X. CARMA3 deficiency abrogates G protein-coupled receptor-induced NF-kappa-B activation. Genes Dev. 21: 984-996, 2007. 3. McAllister-Lucas, L. M., Inohara, N., Lucas, P. C., Ruland, J., Benito, A., Li, Q., Chen, S., Chen, F. F., Yamaoka, S., Verma, I. M., Mak, T. W., Nunez, G. Bimp1, a MAGUK family member linking protein kinase C activation to Bcl10-mediated NF-kappa-B induction. J. Biol. Chem. 276: 30589-30597, 2001. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- CARD10 (Caspase Recruitment Domain Family, Member 10 (CARD10))
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别名
- CARD10
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背景
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Synonyms: CARD10, CARMA3, Caspase recruitment domain-containing protein 10, CARD-containing MAGUK protein 3, Carma 3
Background: Caspase recruitment domain-containing protein 10 is a protein in the CARD-CC protein family that in humans is encoded by the CARD10 gene. The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B through BCL10.
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分子量
- 116 kDa
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基因ID
- 29775
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途径
- S100 Proteins
抗原
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