ANAPC2 抗体 (AA 51-272)
Quick Overview for ANAPC2 抗体 (AA 51-272) (ABIN7601930)
抗原
See all ANAPC2 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 51-272
-
原理
- Anti-ANAPC2 Antibody Picoband®
-
交叉反应 (详细)
- No cross-reactivity with other proteins.
-
产品特性
- Anti-ANAPC2 Antibody Picoband® (ABIN7601930). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human ANAPC2 recombinant protein (Position: K51-R272).
-
亚型
- IgG
-
-
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Dube, P., Herzog, F., Gieffers, C., Sander, B., Riedel, D., Muller, S. A., Engel, A., Peters, J.-M., Stark, H. Localization of the coactivator Cdh1 and the cullin subunit Apc2 in a cryo-electron microscopy model of vertebrate APC/C. Molec. Cell 20: 867-879, 2005. 2. Jorgensen, P. M., Graslund, S., Betz, R., Stahl, S., Larsson, C., Hoog, C. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene 262: 51-59, 2001. 3. Morgan, D., Eley, L., Sayer, J., Strachan, T., Yates, L. M., Craighead, A. S., Goodship, J. A. Expression analyses and interaction with the anaphase promoting complex protein Apc2 suggest a role for inversin in primary cilia and involvement in the cell cycle. Hum. Molec. Genet. 11: 3345-3350, 2002. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- ANAPC2 (Anaphase Promoting Complex Subunit 2 (ANAPC2))
-
别名
- ANAPC2
-
背景
-
Synonyms: S-adenosylmethionine decarboxylase proenzyme, AdoMetDC, SAMDC, 4.1.1.50
Tissue Specificity: Widely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate.
Background: Anaphase-promoting complex subunit 2 is an enzyme that in humans is encoded by the ANAPC2 gene. A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation.
-
分子量
- 110 kDa
-
基因ID
- 29882
-
途径
- Regulation of Cell Size
抗原
-