RPA2 抗体 (AA 34-254)
Quick Overview for RPA2 抗体 (AA 34-254) (ABIN7601380)
抗原
See all RPA2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 34-254
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原理
- Anti-RPA32/RPA2 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-RPA32/RPA2 Antibody Picoband® (ABIN7601380). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human RPA32/RPA2 recombinant protein (Position: Q34-H254).
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亚型
- IgG
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anti-Replication Protein A2, 32kDa (RPA2) (AA 1-270) antibody
RPA2 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Replication Protein A2, 32kDa (RPA2) antibodyRPA2 适用: 人, 犬, 猴 WB, IHC, IF, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 7C12 unconjugated
anti-Replication Protein A2, 32kDa (RPA2) antibodyRPA2 适用: 人, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 1H10 unconjugated
anti-Replication Protein A2, 32kDa (RPA2) antibodyRPA2 适用: 人, 猴 WB, IHC, IF, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 9A1 unconjugated
anti-Replication Protein A2, 32kDa (RPA2) (AA 1-267) antibodyRPA2 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A2, 32kDa (RPA2) antibodyRPA2 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A2, 32kDa (RPA2) (N-Term) antibodyRPA2 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A2, 32kDa (RPA2) (pThr21) antibodyRPA2 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A2, 32kDa (RPA2) (pThr21) antibodyRPA2 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A2, 32kDa (RPA2) (AA 101-200) antibodyRPA2 适用: 小鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chaudhuri, J., Khuong, C., Alt, F. W. Replication protein A interacts with AID to promote deamination of somatic hypermutation targets. Nature 430: 992-998, 2004. 2. Lee, D.-H., Pan, Y., Kanner, S., Sung, P., Borowiec, J. A., Chowdhury, D. A PP4 phosphatase complex dephosphorylates RPA2 to facilitate DNA repair via homologous recombination. Nature Struct. Molec. Biol. 17: 365-372, 2010. 3. Umbricht, C. B., Griffin, C. A., Hawkins, A. L., Grzeschik, K. H., O'Connell, P., Leach, R., Green, E. D., Kelly, T. J. High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3). Genomics 20: 249-257, 1994. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- RPA2 (Replication Protein A2, 32kDa (RPA2))
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别名
- RPA2
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背景
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Synonyms: Interleukin-17F, IL-17F, Il17f,
Background: This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways.
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分子量
- 29 kDa
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基因ID
- 6118
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UniProt
- P15927
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途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
抗原
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