LIG3 抗体 (AA 297-963)
Quick Overview for LIG3 抗体 (AA 297-963) (ABIN7601167)
抗原
See all LIG3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 297-963
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原理
- Anti-DNA Ligase III/LIG3 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-DNA Ligase III/LIG3 Antibody Picoband® (ABIN7601167). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human DNA Ligase III/LIG3 recombinant protein (Position: D297-D963).
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亚型
- IgG
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anti-Ligase III, DNA, ATP-Dependent (LIG3) (AA 730-1009) antibody
LIG3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ligase III, DNA, ATP-Dependent (LIG3) antibodyLIG3 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ligase III, DNA, ATP-Dependent (LIG3) antibodyLIG3 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ligase III, DNA, ATP-Dependent (LIG3) (C-Term) antibodyKO Validated LIG3 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ligase III, DNA, ATP-Dependent (LIG3) (AA 809-928) antibodyLIG3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ligase III, DNA, ATP-Dependent (LIG3) (Internal Region) antibodyLIG3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ligase III, DNA, ATP-Dependent (LIG3) antibodyKO Validated LIG3 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ligase III, DNA, ATP-Dependent (LIG3) (Internal Region) antibodyLIG3 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ligase III, DNA, ATP-Dependent (LIG3) (AA 823-922) antibodyLIG3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Ligase III, DNA, ATP-Dependent (LIG3) (AA 930-979) antibodyLIG3 适用: 人, 小鼠, 大鼠, 犬, 马, 兔, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen, J., Tomkinson, A. E., Ramos, W., Mackey, Z. B., Danehower, S., Walter, C. A., Schultz, R. A., Besterman, J. M., Husain, I. Mammalian DNA ligase III: molecular cloning, chromosomal localization, and expression in spermatocytes undergoing meiotic recombination. Molec. Cell. Biol. 15: 5412-5422, 1995. 2. Gao, Y., Katyal, S., Lee, Y., Zhao, J., Rehg, J. E., Russell, H. R., McKinnon, P. J. DNA ligase III is critical for mtDNA integrity but not Xrcc1-mediated nuclear DNA repair. Nature 471: 240-244, 2011. 3. Moser, J., Kool, H., Giakzidis, I., Caldecott, K., Mullenders, L. H. F., Fousteri, M. I. Sealing of chromosomal DNA nicks during nucleotide excision repair requires XRCC1 and DNA ligase III-alpha in a cell-cycle-specific manner. Molec. Cell 27: 311-323, 2007. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))
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别名
- LIG3
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背景
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Synonyms: Platelet basic protein, PBP, C-X-C motif chemokine 7, Leukocyte-derived growth factor, LDGF, Macrophage-derived growth factor, MDGF, Small-inducible cytokine B7, PPBP, CTAP3, CXCL7, SCYB7, TGB1, THBGB1, NAP-2
Background: DNA ligase 3 is an enzyme that, in humans, is encoded by the LIG3 gene. This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.
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分子量
- 113 kDa
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基因ID
- 3980
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UniProt
- P49916
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途径
- DNA Damage Repair
抗原
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