E2F1 抗体 (AA 230-277)
Quick Overview for E2F1 抗体 (AA 230-277) (ABIN7600771)
抗原
See all E2F1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 230-277
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原理
- Anti-E2F1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-E2F1 Antibody Picoband® (ABIN7600771). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human E2F1 recombinant protein (Position: Q230-D277).
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亚型
- IgG
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anti-E2F Transcription Factor 1 (E2F1) (AA 69-223) antibody
E2F1 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 8G9 unconjugated
anti-E2F Transcription Factor 1 (E2F1) antibodyE2F1 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 兔 Monoclonal 1D12 unconjugated Recombinant Antibody
anti-E2F Transcription Factor 1 (E2F1) (AA 348-437) antibodyE2F1 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2E10 unconjugated
anti-E2F Transcription Factor 1 (E2F1) (AA 101-180) antibodyE2F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (p), IF (cc), FACS, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 1 (E2F1) (AA 133-362) antibodyE2F1 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 1 (E2F1) antibodyE2F1 适用: 人 WB, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 1 (E2F1) (AA 342-371) antibodyE2F1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 1 (E2F1) (pSer364) antibodyE2F1 适用: 人 WB, ELISA, IF (p), IF (cc), FACS, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 1 (E2F1) (pThr433) antibodyE2F1 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 1 (E2F1) (C-Term) antibodyE2F1 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen, D., Pacal, M., Wenzel, P., Knoepfler, P. S., Leone, G., Bremner, R. Division and apoptosis of E2f-deficient retinal progenitors. Nature 462: 925-929, 2009. 2. Chong, J.-L., Wenzel, P. L., Saenz-Robles, M. T., Nair, V., Ferrey, A., Hagan, J. P., Gomez, Y. M., Sharma, N., Chen, H.-Z., Ouseph, M., Wang, S.-H., Trikha, P., and 10 others. E2f1-3 switch from activators in progenitor cells to repressors in differentiating cells. Nature 462: 930-934, 2009. 3. De Angelis, R., Iezzi, S., Bruno, T., Corbi, N., Di Padova, M., Floridi, A., Fanciulli, M., Passananti, C. Functional interaction of the subunit 3 of RNA polymerase II (RPB3) with transcription factor-4 (ATF4). FEBS Lett. 547: 15-19, 2003. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- E2F1 (E2F Transcription Factor 1 (E2F1))
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别名
- E2F1
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背景
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Synonyms: Angiotensin-converting enzyme, ACE, Dipeptidyl carboxypeptidase I, Kininase II, CD143, Angiotensin-converting enzyme, soluble form, Ace, Dcp1
Tissue Specificity: Testis-specific isoform is expressed in spermatocytes, adult testis.
Background: Transcription factor E2F1 is a protein that in humans is encoded by the E2F1 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
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分子量
- 60 kDa
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基因ID
- 1869
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UniProt
- Q01094
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途径
- p53 Pathway, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy
抗原
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