CD14 抗体 (AA 20-259)
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Quick Overview for CD14 抗体 (AA 20-259) (ABIN7600531)
抗原
See all CD14 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 20-259
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原理
- Anti-CD14 Antibody
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-CD14 Antibody (ABIN7600531). Tested in ELISA, Flow Cytometry, IF, IHC, ICC applications. This antibody reacts with Human.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human CD14 recombinant protein (Position: T20-N259).
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亚型
- IgG
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应用备注
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Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- CD14
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别名
- CD14
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背景
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Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, Cd14,
Tissue Specificity: Detected on peritoneal macrophages (at protein level) (PubMed:8612135). Cell surface expression detected in lung alveolar macrophages, dendritic macrophages and lung macrophages (at protein level) (PubMed:19362712).
Background: CD14 is a single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. This gene is located at bands 5q23-q31. The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
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分子量
- 50 kDa
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基因ID
- 929
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UniProt
- P08571
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途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
抗原
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