ITCH 抗体 (AA 17-358)
Quick Overview for ITCH 抗体 (AA 17-358) (ABIN7600263)
抗原
See all ITCH 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 17-358
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原理
- Anti-ITCH/AIP4 Antibody Picoband® (monoclonal, 5E12)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-ITCH/AIP4 Antibody Picoband® (monoclonal, 5E12) (ABIN7600263). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human ITCH/AIP4 recombinant protein (Position: K17-E358).
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亚型
- IgG2b
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anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (Internal Region) antibody
Verified ITCH 适用: 人 ELISA, WB, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) antibodyKO Validated ITCH 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (Tyr420) antibodyITCH 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 29-303) antibodyITCH 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 92-190) antibodyITCH 适用: 人 ELISA, IF 宿主: 小鼠 Monoclonal 1B8 unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 50-150) antibodyKO Validated ITCH 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) antibodyITCH 适用: 人 ELISA, WB 宿主: 兔 Monoclonal 9C3 unconjugated Recombinant Antibody
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (pTyr420) antibodyITCH 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 143-192) antibodyITCH 适用: 人, Cow, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 410-437), (Middle Region) antibodyITCH 适用: 人, 小鼠, 大鼠, 猴 WB, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x6 cells, Human
1. Dorjbal B, Derse D, Lloyd P, Soheilian F, Nagashima K, Heidecker G. ""The role of ITCH protein in human T-cell leukemia virus type 1 release"". J Biol Chem, 2011 Sep 9. 2. Rossi M, Inoue S, Walewska R, Knight RA, Dyer MJ, Cohen GM, Melino G. ""Caspase cleavage of Itch in chronic lymphocytic leukemia cells"". Biochem Biophys Res Commun, 2009 Feb 13. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
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别名
- ITCH
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背景
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Synonyms: Serine/threonine-protein kinase PLK1, Polo-like kinase 1, PLK-1, Serine/threonine-protein kinase 13, STPK13, PLK1, PLK
Tissue Specificity: Placenta and colon.
Background: ITCH is an ubiquitin-conjugating enzyme. This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
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分子量
- 103 kDa
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基因ID
- 83737
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UniProt
- Q96J02
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途径
- Activation of Innate immune Response, CXCR4-mediated Signaling Events
抗原
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