ATG4B 抗体 (AA 17-328)
Quick Overview for ATG4B 抗体 (AA 17-328) (ABIN7600261)
抗原
See all ATG4B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 17-328
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原理
- Anti-ATG4B Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-ATG4B Antibody Picoband® (ABIN7600261). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human ATG4B recombinant protein (Position: E17-D328).
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亚型
- IgG
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anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 1-221) antibody
ATG4B 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4H9A5 unconjugated
anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 1-221) antibodyATG4B 适用: 人, 小鼠 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 6C3C5 unconjugated
anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 350-400) antibodyATG4B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 16-45), (N-Term) antibodyATG4B 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB7496 unconjugated
anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 358-390), (C-Term) antibodyATG4B 适用: 人 WB, IHC (p), IF 宿主: 兔 Polyclonal RB7560 unconjugated
anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 85-200) antibodyATG4B 适用: 人, 小鼠, 大鼠 ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 23-53), (N-Term) antibodyATG4B 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Autophagy related 4B Cysteine Peptidase (ATG4B) antibodyATG4B 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (C-Term) antibodyATG4B 适用: 人, 小鼠, 大鼠, 斑马鱼, 犬, 豚鼠, 兔, 马, 猴, Bat, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 94-106) antibodyATG4B 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Agrotis, A., Pengo, N., Burden, J. J., Ketteler, R. Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Autophagy 15: 976-997, 2019. 2. Gross, M. B. Personal Communication. Baltimore, Md. 4/8/2019. 3. Hemelaar, J., Lelyveld, V. S., Kessler, B. M., Ploegh, H. L. A single protease, Apg4B, is specific for the autophagy-related ubiquitin-like proteins GATE-16, MAP1-LC3, GABARAP, and Apg8L. J. Biol. Chem. 278: 51841-51850, 2003. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))
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别名
- ATG4B
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背景
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Synonyms: Glycerol kinase, GK, Glycerokinase, ATP:glycerol 3-phosphotransferase, GK
Tissue Specificity: Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
Background: Cysteine protease ATG4B is an enzyme that in humans is encoded by the ATG4B gene. Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
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分子量
- 48-50 kDa
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基因ID
- 23192
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UniProt
- Q9Y4P1
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途径
- Autophagy
抗原
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