RAD18 抗体 (AA 1-408)
Quick Overview for RAD18 抗体 (AA 1-408) (ABIN7599364)
抗原
See all RAD18 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-408
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原理
- Anti-RAD18 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-RAD18 Antibody Picoband® (ABIN7599364). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human RAD18 recombinant protein (Position: M1-D408).
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亚型
- IgG
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anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 332-430) antibody
RAD18 适用: 人 WB, ELISA, IF, IHC (p), RNAi 宿主: 小鼠 Monoclonal 3H7 unconjugated
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 1-495) antibodyRAD18 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 332-430) antibodyRAD18 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2G8C10 unconjugated
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 216-495) antibodyRAD18 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (C-Term) antibodyRAD18 适用: 人 WB, ELISA, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 1-495) antibodyRAD18 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (Internal Region) antibodyRAD18 适用: 人, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 1-495) antibodyRAD18 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (Internal Region) antibodyRAD18 适用: 人, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 216-495) antibodyRAD18 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Adams, D. J., van der Weyden, L., Gergely, F. V., Arends, M. J., Ng, B. L., Tannahill, D., Kanaar, R., Markus, A., Morris, B. J., Bradley, A. BRCTx is a novel, highly conserved RAD18-interacting protein. Molec. Cell. Biol. 25: 779-788, 2005. 2. Branzei, D., Vanoli, F., Foiani, M. SUMOylation regulates Rad18-mediated template switch. Nature 456: 915-920, 2008. 3. Centore, R. C., Yazinski, S. A., Tse, A., Zou, L. Spartan/C1orf124, a reader of PCNA ubiquitylation and a regulator of UV-induced DNA damage response. Molec. Cell 46: 625-635, 2012. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))
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别名
- RAD18
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背景
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Synonyms: Guanine deaminase, Guanase, Guanine aminase, Guanine aminohydrolase, GAH, p51-nedasin, GDA, KIAA1258
Tissue Specificity: Expressed in lung carcinoma cell lines but not in normal lung tissues.
Background: E3 ubiquitin-protein ligase RAD18 is an enzyme that in humans is encoded by the RAD18 gene. The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
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分子量
- 75 kDa
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基因ID
- 56852
抗原
-
