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RAD18 抗体 (AA 332-430)

Name: RAD18 抗体 (AA 332-430)
SKU: ABIN566046
Availability: OutOfStock

RAD18 适用: 人 WB, ELISA, IF, IHC (p), RNAi 宿主: 小鼠 Monoclonal 3H7 unconjugated

Western blot analysis of RAD18 over-expressed 293 cell line, cotransfected with RAD18 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1).

Detection limit for recombinant GST tagged RAD18 is approximately 0.

ELISA

Western Blot analysis of RAD18 expression in transfected 293T cell line by RAD18 monoclonal antibody (M01), clone 3H7.

Immunoperoxidase of monoclonal antibody to RAD18 on formalin-fixed paraffin-embedded human testis.

ISt

RAD18 monoclonal antibody (M01), clone 3H7.

Western Blot detection against Immunogen (36.

RAD18 monoclonal antibody (M01), clone 3H7 Western Blot analysis of RAD18 expression in Hela S3 NE .

Immunofluorescence of monoclonal antibody to RAD18 on HeLa cell.

8 images

(7 references)

产品编号 ABIN566046

发货至: 中国

Datasheet as PDF

抗原 See all RAD18 抗体

RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))
抗原表位
7

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 332-430
适用
51

26

22

4

4

3

3

3

2

2

2

1

1
人
宿主
45

6
小鼠
Compatible Secondaries
克隆类型
39

12
单克隆
标记
33

3

2

2

2

2

2

2

2

1
This RAD18 antibody is un-conjugated
应用范围
29

16

9

9

8

3

3

2

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), RNA Interference (RNAi)

原理

Mouse monoclonal antibody raised against a partial recombinant RAD18.

序列

EKEIDEIHSK YRKKHKSEFQ LLVDQARKGY KKIAGMSQKT VTITKEDEST EKLSSVCMGQ EDNMTSVTNH FSQSKLDSPE ELEPDREEDS SSCIDIQEV

交叉反应

人, 大鼠

产品特性

Antibody Reactive Against Recombinant Protein.

免疫原

RAD18 (NP_064550, 332 a.a. ~ 430 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

克隆位点

3H7

亚型

IgG2b
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 1-495) antibody
RAD18 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 332-430) antibody
RAD18 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 2G8C10 unconjugated

anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 216-495) antibody
RAD18 适用: 人 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated

anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (C-Term) antibody
RAD18 适用: 人 WB, ELISA, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 1-495) antibody
RAD18 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-E3 ubiquitin-protein ligase RAD18 (RAD18) antibody
RAD18 适用: 人, 大鼠, 小鼠 IF 宿主: 兔 Polyclonal unconjugated

anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (Internal Region) antibody
RAD18 适用: 人, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 1-495) antibody
RAD18 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (Internal Region) antibody
RAD18 适用: 人, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (C-Term) antibody
RAD18 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
缓冲液

In 1x PBS, pH 7.4

注意事项

Aliquot to avoid repeated freezing and thawing.

储存条件

-20 °C

储存方法

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Sy, Jiang, O, Deng, Huen: "The ubiquitin specific protease USP34 promotes ubiquitin signaling at DNA double-strand breaks." in: Nucleic acids research, Vol. 41, Issue 18, pp. 8572-80, (2013) (PubMed).

Wong, Aguissa-Touré, Wani, Khosravi, Martinka, Martinka, Li: "Elevated expression of Rad18 regulates melanoma cell proliferation." in: Pigment cell & melanoma research, Vol. 25, Issue 2, pp. 213-8, (2012) (PubMed).

Kato, Waki, Umezawa, Aoki, Utsugi, Ohtsu, Murakami: "Phosphorylation of human INO80 is involved in DNA damage tolerance." in: Biochemical and biophysical research communications, Vol. 417, Issue 1, pp. 433-8, (2012) (PubMed).

Wong, Lin, Khosravi, Piche, Jafarnejad, Chen, Li: "Tumour suppressor ING1b maintains genomic stability upon replication stress." in: Nucleic acids research, Vol. 39, Issue 9, pp. 3632-42, (2011) (PubMed).

Yanagihara, Kobayashi, Tateishi, Kato, Matsuura, Tauchi, Yamada, Takezawa, Sugasawa, Masutani, Hanaoka, Weemaes, Mori, Zou, Komatsu: "NBS1 recruits RAD18 via a RAD6-like domain and regulates Pol ?-dependent translesion DNA synthesis." in: Molecular cell, Vol. 43, Issue 5, pp. 788-97, (2011) (PubMed).

Hicks, Chute, Paulsen, Ragland, Howlett, Guéranger, Glover, Canman: "Differential roles for DNA polymerases eta, zeta, and REV1 in lesion bypass of intrastrand versus interstrand DNA cross-links." in: Molecular and cellular biology, Vol. 30, Issue 5, pp. 1217-30, (2010) (PubMed).

Kobayashi, Okui, Asaithamby, Burma, Chen, Tanimoto, Matsuura, Komatsu, Chen: "WRN participates in translesion synthesis pathway through interaction with NBS1." in: Mechanisms of ageing and development, Vol. 131, Issue 6, pp. 436-44, (2010) (PubMed).
抗原

RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

别名

RAD18 (RAD18 产品)

别名

RNF73 antibody, 2810024C04Rik antibody, Rad18sc antibody, zgc:55577 antibody, RAD18 antibody, DDBDRAFT_0218120 antibody, DDBDRAFT_0304878 antibody, DDB_0218120 antibody, DDB_0304878 antibody, DKFZp469H0810 antibody, RAD18, E3 ubiquitin protein ligase antibody, RAD18 E3 ubiquitin protein ligase antibody, E3 ubiquitin-protein ligase RAD18 antibody, E3 ubiquitin-protein ligase antibody, DNA repair protein and ATPase antibody, C3HC4-type zinc finger-containing protein antibody, RAD18 antibody, Rad18 antibody, rad18 antibody

背景

Full Gene Name: RAD18 homolog (S. cerevisiae)
Synonyms: RNF73

基因ID

56852

NCBI登录号

NM_020165