NHEJ1 抗体 (AA 1-273)
Quick Overview for NHEJ1 抗体 (AA 1-273) (ABIN7599198)
抗原
See all NHEJ1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 1-273
-
原理
- Anti-XLF/NHEJ1 Picoband® Antibody
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-XLF/NHEJ1 Picoband® Antibody (ABIN7599198). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human XLF/NHEJ1 recombinant protein (Position: M1-E273).
-
亚型
- IgG
-
-
anti-Nonhomologous End-Joining Factor 1 (NHEJ1) antibody
NHEJ1 适用: 人, 猴 WB, IF, IHC, FACS, IHC (p) 宿主: 小鼠 Monoclonal 3D6 unconjugated
anti-Nonhomologous End-Joining Factor 1 (NHEJ1) antibodyNHEJ1 适用: 人, 大鼠, 小鼠 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nonhomologous End-Joining Factor 1 (NHEJ1) antibodyNHEJ1 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nonhomologous End-Joining Factor 1 (NHEJ1) (AA 1-299) antibodyNHEJ1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Nonhomologous End-Joining Factor 1 (NHEJ1) (C-Term) antibodyVerified NHEJ1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Nonhomologous End-Joining Factor 1 (NHEJ1) (AA 225-296) antibodyNHEJ1 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Nonhomologous End-Joining Factor 1 (NHEJ1) (C-Term) antibodyNHEJ1 适用: 人, 大鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Callebaut, I., Malivert, L., Fischer, A., Mornon, J.-P., Revy, P., de Villartay, J.-P. Cernunnos interacts with the XRCC4-DNA-ligase IV complex and is homologous to the yeast nonhomologous end-joining factor Nej1. J. Biol. Chem. 281: 13857-13860, 2006. 2. Cantagrel, V., Lossi, A.-M., Lisgo, S., Missirian, C., Borges, A., Philip, N., Fernandez, C., Cardoso, C., Figarella-Branger, D., Moncla, A., Lindsay, S., Dobyns, W. B., Villard, L. Truncation of NHEJ1 in a patient with polymicrogyria. Hum. Mutat. 28: 356-364, 2007. 3. Dai, Y., Kysela, B., Hanakahi, L. A., Manolis, K., Riballo, E., Stumm, M., Harville, T. O., West, S. C., Oettinger, M. A., Jeggo, P. A. Nonhomologous end joining and V(D)J recombination require an additional factor. Proc. Nat. Acad. Sci. 100: 2462-2467, 2003. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- NHEJ1 (Nonhomologous End-Joining Factor 1 (NHEJ1))
-
别名
- NHEJ1
-
背景
-
Synonyms: Non-homologous end-joining factor 1, Protein cernunnos, XRCC4-like factor, NHEJ1, XLF
Tissue Specificity: Widely expressed.
Background: Non-homologous end-joining factor 1 (NHEJ1), also known as Cernunnos or XRCC4-like factor (XLF), is a protein that in humans is encoded by the NHEJ1 gene. It is mapped to 2q35. Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.
-
分子量
- 38 kDa
-
基因ID
- 79840
-
UniProt
- Q9H9Q4
-
途径
- DNA Damage Repair
抗原
-
