NHEJ1 抗体 (AA 1-273)
Quick Overview for NHEJ1 抗体 (AA 1-273) (ABIN7599198)
抗原
See all NHEJ1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-273
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原理
- Anti-XLF/NHEJ1 Antibody
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-XLF/NHEJ1 Antibody (ABIN7599198). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human XLF/NHEJ1 recombinant protein (Position: M1-E273).
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亚型
- IgG
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- NHEJ1 (Nonhomologous End-Joining Factor 1 (NHEJ1))
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别名
- NHEJ1
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背景
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Synonyms: Non-homologous end-joining factor 1, Protein cernunnos, XRCC4-like factor, NHEJ1, XLF
Tissue Specificity: Widely expressed.
Background: Non-homologous end-joining factor 1 (NHEJ1), also known as Cernunnos or XRCC4-like factor (XLF), is a protein that in humans is encoded by the NHEJ1 gene. It is mapped to 2q35. Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.
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分子量
- 38 kDa
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基因ID
- 79840
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UniProt
- Q9H9Q4
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途径
- DNA Damage Repair
抗原
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