我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

MEK2 抗体 (AA 1-50)

Name: MEK2 抗体 (AA 1-50)
SKU: ABIN726500
Availability: OutOfStock
Rating: 5 (1 reviews)

This 兔多克隆 anti-MEK2 antibody specifically detects MEK2 in WB, ELISA, IHC (p), FACS, IF (cc), IF (p) 和 IHC (fro). The antibody is reactive with 人和小鼠 samples. Independently validated for use in Western Blotting.

Western Blotting validation image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500) (MEK2 抗体 (AA 1-50))

Images provided by the Independent Validation Program, badge number 029748. (MEK2 抗体 (AA 1-50))

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti MEK2/MAPKK2 Polyclonal Antibody, Unconjugated (ABIN726500) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (MEK2 抗体 (AA 1-50))

IHC

HT-55 cells were stained with bs-0223R Rabbit Anti-MEK2 Polyclonal Antibody at 1:250. (MEK2 抗体 (AA 1-50))

IF (cc)

4 images

(1 validation)

产品编号 ABIN726500

发货至: 中国

Contact our Customer Service for availability and price in your country. Contact Info

Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for MEK2 抗体 (AA 1-50) (ABIN726500)

抗原

See all MEK2 (MAP2K2) 抗体

MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

适用

人, 小鼠

宿主

兔

克隆类型

多克隆

标记

This MEK2 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

抗原表位
18

15

8

8

7

7

7

6

6

5

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-50

交叉反应

人, 小鼠

预测反应

Rat,Chicken

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human MAPKK2/MEK2

亚型

IgG
anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 44-313) antibody
MAP2K2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-100) antibody
KO Validated MAP2K2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-30), (N-Term) antibody
MAP2K2 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal RB3595 unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-400) antibody
MAP2K2 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-360) antibody
MAP2K2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 8D10 unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 200-229) antibody
MAP2K2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB12322 unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 262-292) antibody
MAP2K2 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB3597 unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 264-400) antibody
MAP2K2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (pThr394) antibody
MAP2K2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 372-400) antibody
MAP2K2 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB14015 unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
生效 #029748 (Western Blotting)

by

Alamo Laboratories Inc

No.

#029748

日期

2014.07.02

抗原

Lot Number

110511

Method validated

Western Blotting

Positive Control

Hela cell extract

Negative Control

c6/36 Mosquito cell extract (non-reactive species)

Notes

A single positive band at the correct molecular weight was detected in positive control HeLa cell extract. Several bands were observed in the non-reactive species negative control at approximately the same molecular weight as MAP2K2. These bands may constitute cross-reactivity of the target antigen, or they may be non-specific bands.

Validation Images

Figure 1. Western blot of lysates from HeLa cells (Lane 1) and c6/36 cells (Lane 2) probed with anti-MAP2K2 (upper panel) or with anti-Actin for loading control (lower panel).
Full Methods
Primary Antibody

Antigen: Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (1:150 dilution)
Catalog number: ABIN726500
Lot number: 110511

Secondary Antibody

Antibody: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:20,000 dilution)
Lot number: L170-6515

Full Protocol

1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
2. 46 μg of boiled extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
5. The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) non-fat dry milk at room temperature for 1 h.
6. The membrane was rinsed with TBST once.
7. The membrane was immersed with the protein side up in the primary antibody solution (anti-MAP2K2; 1:150) in TBST containing 5% (W/V) non-fat dry milk and incubated for 16 h at 4°C.
8. The membrane was rinsed in TBST thrice for 5 min each.
9. The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) non-fat dry milk and incubated for 1 h at room temperature (~26°C) with gentle agitation.
10. The membrane was rinsed thrice TBST thrice for 5 min each.
11. The membrane was rinsed in TBS twice for 30 s each.
12. Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
13. The membrane was rinsed three times TBST.
14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
15. The membrane was washed in TBST 2 times for 10 min each.
16. Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).

Experimental Notes

- No challenges noted.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

别名

MEK2

背景

Synonyms: CFC4, MEK2, MKK2, MAPKK2, PRKMK2, Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2, MAP2K2
Background: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).

基因ID

5605

UniProt

P36507

途径

MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling