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MEK2 抗体 (AA 1-50)

Name: MEK2 抗体 (AA 1-50)
SKU: ABIN726500
Availability: OutOfStock
Rating: 5 (1 reviews)

This anti-MEK2 antibody is a 兔多克隆 antibody detecting MEK2 in WB, ELISA, IHC (p), FACS, IF (cc), IF (p) 和 IHC (fro). Suitable for 人和小鼠. Independently validated for use in Western Blotting.

Western Blotting validation image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500) (MEK2 抗体 (AA 1-50))

Images provided by the Independent Validation Program, badge number 029748. (MEK2 抗体 (AA 1-50))

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti MEK2/MAPKK2 Polyclonal Antibody, Unconjugated (ABIN726500) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (MEK2 抗体 (AA 1-50))

IHC

HT-55 cells were stained with bs-0223R Rabbit Anti-MEK2 Polyclonal Antibody at 1:250. (MEK2 抗体 (AA 1-50))

IF (cc)

4 images

(1 validation)

产品编号 ABIN726500

发货至: 中国

Datasheet as PDF

Quick Overview for MEK2 抗体 (AA 1-50) (ABIN726500)

抗原

See all MEK2 (MAP2K2) 抗体

MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

适用

人, 小鼠

宿主

兔

克隆类型

多克隆

标记

This MEK2 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

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AA 1-50

交叉反应

人, 小鼠

预测反应

Rat,Chicken

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human MAPKK2/MEK2

亚型

IgG
anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 44-313) antibody
MAP2K2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-30), (N-Term) antibody
MAP2K2 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal RB3595 unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-400) antibody
MAP2K2 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-360) antibody
MAP2K2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 8D10 unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-100) antibody
KO Validated MAP2K2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 200-229) antibody
MAP2K2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB12322 unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 262-292) antibody
MAP2K2 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB3597 unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 264-400) antibody
MAP2K2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (C-Term) antibody
MAP2K2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (pThr394) antibody
MAP2K2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
生效 #029748 (Western Blotting)

by

Alamo Laboratories Inc

No.

#029748

日期

2014.07.02

抗原

Lot Number

110511

Method validated

Western Blotting

Positive Control

Hela cell extract

Negative Control

c6/36 Mosquito cell extract (non-reactive species)

Notes

A single positive band at the correct molecular weight was detected in positive control HeLa cell extract. Several bands were observed in the non-reactive species negative control at approximately the same molecular weight as MAP2K2. These bands may constitute cross-reactivity of the target antigen, or they may be non-specific bands.

Validation Images

Figure 1. Western blot of lysates from HeLa cells (Lane 1) and c6/36 cells (Lane 2) probed with anti-MAP2K2 (upper panel) or with anti-Actin for loading control (lower panel).
Full Methods
Primary Antibody

Antigen: Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (1:150 dilution)
Catalog number: ABIN726500
Lot number: 110511

Secondary Antibody

Antibody: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:20,000 dilution)
Lot number: L170-6515

Full Protocol

1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
2. 46 μg of boiled extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
5. The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) non-fat dry milk at room temperature for 1 h.
6. The membrane was rinsed with TBST once.
7. The membrane was immersed with the protein side up in the primary antibody solution (anti-MAP2K2; 1:150) in TBST containing 5% (W/V) non-fat dry milk and incubated for 16 h at 4°C.
8. The membrane was rinsed in TBST thrice for 5 min each.
9. The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) non-fat dry milk and incubated for 1 h at room temperature (~26°C) with gentle agitation.
10. The membrane was rinsed thrice TBST thrice for 5 min each.
11. The membrane was rinsed in TBS twice for 30 s each.
12. Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
13. The membrane was rinsed three times TBST.
14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
15. The membrane was washed in TBST 2 times for 10 min each.
16. Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).

Experimental Notes

- No challenges noted.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

别名

MEK2

背景

Synonyms: CFC4, MEK2, MKK2, MAPKK2, PRKMK2, Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2, MAP2K2
Background: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).

基因ID

5605

UniProt

P36507

途径

MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling