IGSF8 抗体 (Extracellular)
Quick Overview for IGSF8 抗体 (Extracellular) (ABIN7581902)
抗原
See all IGSF8 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 227-242, Extracellular
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原理
- A Rabbit polyclonal antibody to IGSF8 (extracellular)
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序列
- (C)EAGAPYAERL ASGELR
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特异性
- Extracellular, N-terminus.
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预测反应
- Rat-14,Human-15,16 identical
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产品特性
- Anti-IGSF8 (extracellular) Antibody (ABIN7581902) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize IGSF8 from mouse, rat and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)EAGAPYAERLASGELR, corresponding to amino acid residues 227 - 242 of mouse IGSF8
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亚型
- IgG
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anti-Immunoglobulin Superfamily, Member 8 (IGSF8) (AA 220-322) antibody
IGSF8 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Immunoglobulin Superfamily, Member 8 (IGSF8) (Internal Region) antibodyIGSF8 适用: 人, Cow, Pig, 兔, 猴, Bat IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Immunoglobulin Superfamily, Member 8 (IGSF8) antibodyIGSF8 适用: 人 FACS, IHC (fro), IP 宿主: 小鼠 Monoclonal 8A12 unconjugated
anti-Immunoglobulin Superfamily, Member 8 (IGSF8) antibody (PE)IGSF8 适用: 人 FACS 宿主: 小鼠 Monoclonal 8A12 PE
anti-Immunoglobulin Superfamily, Member 8 (IGSF8) (AA 226-254) antibodyIGSF8 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB31348 unconjugated
anti-Immunoglobulin Superfamily, Member 8 (IGSF8) (AA 220-322) antibodyIGSF8 适用: 人 ELISA 宿主: 小鼠 Monoclonal 1E4 unconjugated
anti-Immunoglobulin Superfamily, Member 8 (IGSF8) (AA 28-350) antibodyIGSF8 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Immunoglobulin Superfamily, Member 8 (IGSF8) (Middle Region) antibodyIGSF8 适用: 人, 小鼠, Cow, Pig, 兔, 犬, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Immunoglobulin Superfamily, Member 8 (IGSF8) (AA 233-262), (Middle Region) antibodyIGSF8 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Antigen preadsorption control: 1 μg peptide per 1 μg antibody
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- IGSF8 (Immunoglobulin Superfamily, Member 8 (IGSF8))
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别名
- IGSF8
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背景
- Immunoglobulin superfamily member 8, Prostaglandin Regulatory-Like Protein, PGRL, Glu-Trp-Ile EWI motif-containing protein 2, EWI2, CD81 Partner 3, KCT4, CD316,IGSF8, also referred to as EWI-2, CD316, and PGRL, is a member of the immunoglobulin superfamily (IgSF), which is characterized by extracellular immunoglobulin-like domains. IGSF8 specifically contains four such domains along with a short cytoplasmic tail, enabling its roles in cell-cell adhesion, intracellular signaling, and the organization of cell surface microdomains1,2. It associates with tetraspanins, such as CD9 and CD81, forming the tetraspanin web, a molecular network that organizes cell-surface proteins into functional microdomains and regulates processes like adhesion, migration, and signaling3. Furthermore, IGSF8 interacts with the ezrin-radixin-moesin (ERM) protein family, linking the tetraspanin web to the actin cytoskeleton and contributing to cell polarity and motility4,5. Functionally, IGSF8 acts as an immune checkpoint by suppressing natural killer (NK) cell cytotoxicity through interactions with receptors like KIR3DL2, allowing tumor cells to evade immune detection3. Its expression correlates with poor outcomes in cancer and low antigen presentation, making it a promising target for therapeutic intervention3,4. Beyond its role in immune modulation, IGSF8 contributes to reproductive biology by participating in sperm-egg fusion2. IGSF8 is expressed across various tissues, including neural and immune cells1. Disruption of its function enhances cellular migration and inhibits metastasis4, highlighting its therapeutic relevance. As a cancer immunotherapy target, IGSF8 has shown potential, particularly when combined with immune checkpoint inhibitors like anti-PD1 antibodies3. Research into IGSF8 is critical for understanding tumor immune evasion, immunotherapeutic development, and its roles in neural and reproductive biology1,5.
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基因ID
- 140559
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UniProt
- Q8R366
抗原
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