RFP 抗体
Quick Overview for RFP 抗体 (ABIN7565987)
抗原
See all RFP 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-401-379
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Supplier
- Rockland
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原理
- RFP Antibody Pre-adsorbed
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交叉反应 （详细）
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Minimal crossreactivity against Hu Ms & Rt Serum Proteins
Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. -
产品特性
- Synonyms: rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583.
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纯化方法
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Preadsorption: Pre-Adsorbed
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过滤
- Sterile filtered
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免疫原
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Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.
Immunogen Type: Recombinant Protein
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anti-Red Fluorescent Protein (RFP) antibody
RFP 适用: Discosoma WB, ELISA, IF, IHC, IP, FACS, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Red Fluorescent Protein (RFP) antibodyRFP 适用: Discosoma WB, ELISA, IP, FACS, FM 宿主: 小鼠 Monoclonal 8E5-G7 unconjugated
anti-Red Fluorescent Protein (RFP) antibodyRFP 适用: Discosoma WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Red Fluorescent Protein (RFP) antibodyRFP 适用: Discosoma WB, ELISA 宿主: 小鸡 Polyclonal unconjugated
anti-Red Fluorescent Protein (RFP) antibody (DyLight 550)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550
anti-Red Fluorescent Protein (RFP) antibody (DyLight 633)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 633
anti-Red Fluorescent Protein (RFP) antibody (DyLight 488)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 488
anti-Red Fluorescent Protein (RFP) antibody (Biotin)RFP 适用: Discosoma WB, ELISA, IHC 宿主: 兔 Polyclonal Biotin
anti-Red Fluorescent Protein (RFP) antibody (Biotin)RFP 适用: Discosoma WB, ELISA, IF, FM 宿主: 小鸡 Polyclonal Biotin
anti-Red Fluorescent Protein (RFP) (N-Term) antibodyRFP 适用: Discosoma WB, ELISA, IF, IHC, IP, DB 宿主: 小鼠 Monoclonal RF5R unconjugated
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应用备注
- Application Note: RFP Antibody Pre-adsorbed is designed for detecting RFP and its variants, tested across ELISA, Western blot, IF, IHC, and suitable for EM, FC, FISH, IP, and multiplex assays. It is applicable for ELISA in both sandwich and capture formats. Researchers should determine optimal titers for specific applications. Recommended starting dilutions are: ELISA 1:28,700 - 1:48,700, FC, IF, IHC 1:200 - 1:2,000, WB 1:1,000 - 1:5,000. Flow Cytometry Dilution: 1:200 - 1:2,000 Immunohistochemistry Dilution: 1:200 - 1:2,000 Western Blot Dilution: 1:1,000 - 1:5,000 Immunoprecipitation Dilution: User Optimized ELISA Dilution: 1:28,700 - 1:48,700 IF Microscopy Dilution: 1:200 - 1:2,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.17 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- RFP (Red Fluorescent Protein (RFP))
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别名
- DsRed
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背景
- Background: RFP Antibody Pre-adsorbed is a high-quality, rabbit polyclonal antibody specifically designed for the detection of red fluorescent protein (RFP) and its variants like mCherry, mScarlet, and tdTomato. This product is suitable for a broad range of applications including Western blot (WB), ELISA, immunohistochemistry (IHC), immunofluorescence (IF), flow cytometry (FC), and more. It is uniquely pre-adsorbed to minimize cross-reactivity, offering high specificity and sensitivity. With over 900 references, this product stands as a proven tool in the scientific community, supporting a wide range of research studies from cellular signaling to disease pathology.
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UniProt
- Q9U6Y8
抗原
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