SIRT1 抗体 (C-Term)
Quick Overview for SIRT1 抗体 (C-Term) (ABIN7565978)
抗原
See all SIRT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- SIRT1 Antibody
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交叉反应 （详细）
- Anti-SIRT1 is directed against human SIRT1.
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产品特性
- Synonyms: rabbit anti-SIRT1 antibody, NAD-dependent protein deacetylase sirtuin-1, hSIRT1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, hSIR2, SirtT1 75 kDa fragment, 75SirT1, SIRT-1, SIRT 1, SIR2L1
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纯化方法
- This product is an affinity purified antibody produced by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Affinity purified Anti-SIRT1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the c-terminal of human SIRT1 protein.
Immunogen Type: Conjugated Peptide
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anti-Sirtuin 1 (SIRT1) (AA 101-200) antibody
SIRT1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Sirtuin 1 (SIRT1) (AA 456-555) antibodySIRT1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 7B7 unconjugated
anti-Sirtuin 1 (SIRT1) (AA 448-747) antibodySIRT1 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Sirtuin 1 (SIRT1) (AA 566-601), (C-Term) antibodySIRT1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB50965 unconjugated
anti-Sirtuin 1 (SIRT1) antibodySIRT1 适用: 人 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1F3 unconjugated
anti-Sirtuin 1 (SIRT1) antibodyKO Validated SIRT1 适用: 人 WB, IHC, IP, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Sirtuin 1 (SIRT1) (N-Term) antibodySIRT1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 豚鼠, 马, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Sirtuin 1 (SIRT1) (AA 566-601) antibodySIRT1 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Sirtuin 1 (SIRT1) (AA 456-555) antibodySIRT1 适用: 人 WB, IF, IHC (p), EIA 宿主: 小鼠 Monoclonal 7B7 unconjugated
anti-Sirtuin 1 (SIRT1) (pSer47) antibodySIRT1 适用: 人, 小鼠, 大鼠, 犬 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~62 or 81.7 kDa in size corresponding to SIRT1 by western blotting in the appropriate cell lysate or extract. Higher observed band ~125 kda is due to complexing proteins. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 5.0 μg/mL
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.07 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- SIRT1 (Sirtuin 1 (SIRT1))
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别名
- SIRT1
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背景
- Background: SIRT1 antibody detects human SIRT1 protein. SIRT1 is a member of the sirtuin family of protein-modifying enzymes. SIRT1 is a NAD+-dependent deacetylase that plays an important role in many cellular processes. SIRT1 protein is known to regulate epigenetic gene silencing and suppress recombination of rDNA. SIRT1 deacetylates a wide range of substrates, including p53, NF-kB, FOXO transcription factors, and PGC-1 alpha, with roles in cellular processes such as muscle differentiation, adipogenesis, protection from axonal degeneration, and life span extension. SIRT1 is downregulated in cells that have high insulin resistance and inducing its expression increases insulin sensitivity, suggesting the molecule is associated with improving insulin sensitivity. Furthermore, SIRT1 de-acetylates and affects the activity of both members of the PGC1-alpha/ERR-alpha complex, which are essential metabolic regulatory transcription factors. Anti-SIRT1 Antibody is useful for researcher involved in research areas including cancer, diabetes, aging, neurodegenerative, metabolic or cardiovascular diseases.
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基因ID
- 23411
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NCBI登录号
- NP_001135970
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UniProt
- Q96EB6
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途径
- MAPK Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling
抗原
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