NRF2 抗体 (C-Term)
Quick Overview for NRF2 抗体 (C-Term) (ABIN7565937)
抗原
See all NRF2 (NFE2L2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Nrf2 Antibody
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交叉反应 (详细)
- This affinity-purified antibody is directed against mouse Nrf2 protein.
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产品特性
- Synonyms: rabbit anti-Nrf2 antibody, Nrf 2, Nrf-2 Antibody, Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, Nuclear factor, erythroid derived 2, Nfe2l2, HEBP1
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-NRF-2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding near the c-term region.
Immunogen Type: Conjugated Peptide
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应用备注
- Application Note: This affinity purified Nrf2 antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 66.9 kDa in size corresponding to Nrf-2 proteins by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 5 μg/mL
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))
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别名
- Nfe2l2
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背景
- Background: Anti-Nrf-2 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Nrf2 links the transcriptional modulation of key metabolic genes to cellular growth and development and has been implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. Nrf2 is localized in the cytoplasm and nucleus and ubiquitously expressed with the highest concentrations in the kidney, muscle, lung, heart, liver, and brain. Anti-Nrf2 antibody is useful for researchers interested in cellular growth, transcription factor, and cancer research.
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基因ID
- 18024
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NCBI登录号
- NP_035032
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UniProt
- Q60795
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途径
- ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling
抗原
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