Cyclin D3 抗体 (C-Term)
Quick Overview for Cyclin D3 抗体 (C-Term) (ABIN7565932)
抗原
See all Cyclin D3 (CCND3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Cyclin D3 Antibody
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交叉反应 （详细）
- Cyclin D3 affinity-purified antibody is directed against Cyclin D3 protein.
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产品特性
- Synonyms: rabbit anti-Cyclin D3 antibody, G1/S-specific cyclin-D3, Ccnd3, Cyl-3
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of mouse Cyclin D3 protein.
Immunogen Type: Conjugated Peptide
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anti-Cyclin D3 (CCND3) (AA 1-292) antibody
CCND3 适用: 人 WB, ELISA, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D3 (CCND3) (pThr283) antibodyCCND3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D3 (CCND3) (AA 136-292) antibodyCCND3 适用: 人, 大鼠 WB, FACS 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D3 (CCND3) (AA 121-220) antibodyCCND3 适用: 人, 兔 WB, ELISA, IF (cc), IF (p), FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D3 (CCND3) (AA 1-292) antibodyCCND3 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D3 (CCND3) (AA 1-292) antibodyCCND3 适用: 人 WB, IHC (p), IF 宿主: 小鼠 Polyclonal unconjugated
anti-Cyclin D3 (CCND3) (C-Term) antibodyCCND3 适用: 人, 小鼠, 大鼠, Cow, 犬, Pig, 绵羊, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D3 (CCND3) (C-Term) antibodyCCND3 适用: 人, 小鼠 WB, ELISA, IHC, FACS, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D3 (CCND3) antibodyCCND3 适用: 人 WB, IF (cc) 宿主: 兔 Monoclonal 4A8 unconjugated
anti-Cyclin D3 (CCND3) (AA 1-292) antibodyCCND3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, flow cytometry, and by immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 32.4 kDa in size corresponding to Cyclin D3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. This reagent reacts equally to phosphorylated and non-phosphorylated Cyclin D3 by ELISA and western blotting. Flow Cytometry Dilution: 4 μg/mL Immunohistochemistry Dilution: 4 μg/mL Western Blot Dilution: 1.0 μg/mL Immunoprecipitation Dilution: 5 μg/mL ELISA Dilution: 1:1000-1:5000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Cyclin D3 (CCND3)
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别名
- Ccnd3
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背景
- Background: Anti-Cyclin D3 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Cyclin D3 belongs to a highly conserved cyclin family, whose members are the ultimate recipients of oncogenic signals. Cyclin D3 is a key component of the cell cycle progression machinery and induces progression through the G1 phase of the cell cycle. Cyclin D3 is expressed in nearly all proliferating cells, and shows the most broad expression pattern of all three D-type (D1-D3) cyclins. Cyclin D3 is encoded from the 6p21 chromosome region and the protein is predominantly localized in the nucleus. Once induced, cyclin D3 binds and activates its associated cyclin-dependent kinases CDK4 and CDK6. Amplification of the cyclin D3 gene and overexpression of cyclin D3 protein is seen in several human cancers. A large number of human malignancies contain lesions in pathways impacting on cyclin D3. Abnormal expression of Cyclin D3 is believed to be a driving force in several human cancers. A possible role for cyclin D3 in the malignancies of the lymphoid system is suggested by the observations that cyclin D3 gene is rearranged in several neoplastic diseases such as diffuse large B cell lymphomas or multiple myelomas. Anti-Cyclin D3 is ideal for researchers interested in Cancer Research and Immunology research.
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基因ID
- 12445
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UniProt
- P30282
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, Glycosaminoglycan Metabolic Process
抗原
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