Cyclin D3 抗体 (C-Term)
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- 抗原 See all Cyclin D3 (CCND3) 抗体
- Cyclin D3 (CCND3)
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抗原表位
- C-Term
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Cyclin D3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)
- 产品特性
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Synonyms: rabbit anti-Cyclin D3 antibody, G1/S-specific cyclin-D3, Ccnd3, Cyl-3
Background: Anti-Cyclin D3 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Cyclin D3 belongs to a highly conserved cyclin family, whose members are the ultimate recipients of oncogenic signals. Cyclin D3 is a key component of the cell cycle progression machinery and induces progression through the G1 phase of the cell cycle. Cyclin D3 is expressed in nearly all proliferating cells, and shows the most broad expression pattern of all three D-type (D1-D3) cyclins. Cyclin D3 is encoded from the 6p21 chromosome region and the protein is predominantly localized in the nucleus. Once induced, cyclin D3 binds and activates its associated cyclin-dependent kinases CDK4 and CDK6. Amplification of the cyclin D3 gene and overexpression of cyclin D3 protein is seen in several human cancers. A large number of human malignancies contain lesions in pathways impacting on cyclin D3. Abnormal expression of Cyclin D3 is believed to be a driving force in several human cancers. A possible role for cyclin D3 in the malignancies of the lymphoid system is suggested by the observations that cyclin D3 gene is rearranged in several neoplastic diseases such as diffuse large B cell lymphomas or multiple myelomas. Anti-Cyclin D3 is ideal for researchers interested in Cancer Research and Immunology research.
Gene Name: Ccnd3
- 纯化方法
- Cyclin D3 affinity-purified antibody is directed against Cyclin D3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse, human, and bovine sources based on 100% homology for the immunogen sequence. Cross reactivity with other sources has not been determined. No reactivity was observed against Cyclin D2.
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of mouse Cyclin D3 protein.
Immunogen Type: Peptide
- 亚型
- IgG
- Top Product
- Discover our top product CCND3 Primary Antibody
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anti-Cyclin D3 (CCND3) (AA 121-220) antibody
CCND3 适用: 人, 兔 WB, ELISA, IF (cc), IHC (p), IF (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D3 (CCND3) (AA 1-292) antibodyCCND3 适用: 人 WB, ELISA, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D3 (CCND3) (pThr283) antibodyCCND3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D3 (CCND3) (AA 136-292) antibodyCCND3 适用: 人, 大鼠 WB, FACS 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D3 (CCND3) (AA 1-292) antibodyCCND3 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D3 (CCND3) (AA 1-292) antibodyCCND3 适用: 人 WB, IHC (p), IF 宿主: 小鼠 Polyclonal unconjugated
anti-Cyclin D3 (CCND3) (C-Term) antibodyCCND3 适用: 人, 小鼠, 大鼠, Cow, 犬, Pig, 绵羊, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D3 (CCND3) antibodyCCND3 适用: 人 WB, IF (cc) 宿主: 兔 Monoclonal 4A8 unconjugated
anti-Cyclin D3 (CCND3) (AA 1-292) antibodyCCND3 适用: 人 WB, ELISA, IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D3 (CCND3) (AA 33-66), (N-Term) antibodyCCND3 适用: 大鼠 WB 宿主: 兔 Polyclonal RB43404 unconjugated
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- 应用备注
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Flow Cytometry Dilution: 4 μg/mL
Immunohistochemistry Dilution: 4 μg/mL
Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, flow cytometry, and by immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 32.4 kDa in size corresponding to Cyclin D3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated Cyclin D3.
Western Blot Dilution: 1.0 μg/mL
Immunoprecipitation Dilution: 5 μg/mL
ELISA Dilution: 1:1000 - 1:5000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- Cyclin D3 (CCND3)
- 别名
- Cyclin D3 (CCND3 产品)
- 别名
- zgc:103502 antibody, CCND3 antibody, 9230106B05Rik antibody, AA682053 antibody, AL024085 antibody, AW146355 antibody, C78795 antibody, cyclin D3 antibody, ccnd3 antibody, CCND3 antibody, Ccnd3 antibody
- 基因ID
- 12445
- UniProt
- P30282
- 途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, Glycosaminoglycan Metabolic Process
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