RFP 抗体
Quick Overview for RFP 抗体 (ABIN7565910)
抗原
See all RFP 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Ready-To-Use RFP Antibody Pre-adsorbed
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交叉反应 （详细）
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Minimal crossreactivity against Hu Ms & Rt Serum Proteins
Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. -
产品特性
- Synonyms: DsRed protein, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583, sea anemone Discosoma sp. Mushroom, RFP antibody, RFP-RTU, Ready To Use Antibody, RTU Antibody
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纯化方法
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RTU Anti-RFP was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Preadsorption: Pre-Adsorbed
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过滤
- Sterile filtered
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免疫原
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Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.
Immunogen Type: Recombinant Protein
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anti-Red Fluorescent Protein (RFP) antibody
RFP 适用: Discosoma WB, ELISA, IF, IHC, IP, FACS, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Red Fluorescent Protein (RFP) antibodyRFP 适用: Discosoma WB, ELISA, IP, FACS, FM 宿主: 小鼠 Monoclonal 8E5-G7 unconjugated
anti-Red Fluorescent Protein (RFP) antibodyRFP 适用: Discosoma WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Red Fluorescent Protein (RFP) antibodyRFP 适用: Discosoma WB, ELISA 宿主: 小鸡 Polyclonal unconjugated
anti-Red Fluorescent Protein (RFP) antibody (DyLight 550)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550
anti-Red Fluorescent Protein (RFP) antibody (DyLight 633)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 633
anti-Red Fluorescent Protein (RFP) antibody (DyLight 488)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 488
anti-Red Fluorescent Protein (RFP) antibody (Biotin)RFP 适用: Discosoma WB, ELISA, IHC 宿主: 兔 Polyclonal Biotin
anti-Red Fluorescent Protein (RFP) antibody (Biotin)RFP 适用: Discosoma WB, ELISA, IF, FM 宿主: 小鸡 Polyclonal Biotin
anti-Red Fluorescent Protein (RFP) (N-Term) antibodyRFP 适用: Discosoma WB, ELISA, IF, IHC, IP, DB 宿主: 小鼠 Monoclonal RF5R unconjugated
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应用备注
- Application Note: Ready-To-Use Anti-RFP is designed to detect RFP and its variants. Ready-To-Use Anti-RFP Rabbit Polyclonal Antibody has been optimized and tested in ELISA and in Western blot at a 1:1000 dilution. This Anti-RFP (RTU) Antibody is sufficient for 10 Western blots. Although not tested, there's a high likelihood that this antibody is functional in immunohistochemistry, immunofluorescence, and immunoprecipitation. Researchers can determine the best concentrations for these applications based on their specific experiments. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:1,000 Immunoprecipitation Dilution: User Optimized ELISA Dilution: User Optimized IF Microscopy Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.005 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.01 % Bovine Serum Albumin (BSA), 25 % (v/v) Glycerol
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 2-8° C prior to opening. May aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to use.
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有效期
- 12 months
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- RFP (Red Fluorescent Protein (RFP))
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别名
- DsRed
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背景
- Background: Fluorescent proteins like green fluorescent protein (GFP) and Discosoma Red Fluorescent Protein (DsRFP) are widely used in research. DsRFP is derived from the mushroom anemone Discosoma sp. Despite sharing only 19 % similarity, anti-GFP antibodies typically don't recognize DsRFP and vice versa. Structurally, DsRFP is akin to GFP in its overall β-can fold and chromophore-formation chemistry. However, GFP undergoes an extra step in chromophore maturation and maintains a tetrameric structure. By using site-directed mutagenesis, various DsRFP variants have been engineered, enabling the red fluorescent protein to mature as a monomer. These monomeric DsRFP variants-mRFP1, mBanana, mCherry, mHoneydew, mPlum, mOrange, mStrawberry, and mTangerine-provide a diverse range of fluorescent colors. Rockland's RFP polyclonal antibodies, raised against the entire wild-type RFP protein, are expected to recognize all these variant forms. These antibodies have been pre-absorbed to prevent potential cross-reactivity with human, mouse, and rat serum proteins, and have also been confirmed to not react with GFP protein. All Rockland Immunochemical's RFP antibodies undergo stringent affinity purification to ensure high specificity and affinity. Thorough quality control tests guarantee that the final product meets or exceeds the highest standards, ensuring optimal performance in your assays.
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UniProt
- Q9U6Y8
抗原
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