RFP 抗体
Quick Overview for RFP 抗体 (ABIN7565893)
抗原
See all RFP 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-mCherry Antibody Pre-Adsorbed
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交叉反应 （详细）
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Minimal crossreactivity against Hu Ms & Rt Serum Proteins
Expect reactivity against mCherry, RFP and its variants: tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. -
产品特性
- Synonyms: rabbit anti-mCherry antibody, RFP, mCherry monomeric red fluorescent protein, Red Fluorescent Protein (RFP), rDsRed, Discosoma sp. Red Fluorescent Protein
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纯化方法
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mCherry was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Preadsorption: Pre-Adsorbed
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: The immunogen is a mCherry mutant variant fusion protein of RFP corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
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anti-Red Fluorescent Protein (RFP) antibody
RFP 适用: Discosoma WB, ELISA, IF, IHC, IP, FACS, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Red Fluorescent Protein (RFP) antibodyRFP 适用: Discosoma WB, ELISA, IP, FACS, FM 宿主: 小鼠 Monoclonal 8E5-G7 unconjugated
anti-Red Fluorescent Protein (RFP) antibodyRFP 适用: Discosoma WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Red Fluorescent Protein (RFP) antibodyRFP 适用: Discosoma WB, ELISA 宿主: 小鸡 Polyclonal unconjugated
anti-Red Fluorescent Protein (RFP) antibody (DyLight 550)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550
anti-Red Fluorescent Protein (RFP) antibody (DyLight 633)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 633
anti-Red Fluorescent Protein (RFP) antibody (DyLight 488)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 488
anti-Red Fluorescent Protein (RFP) antibody (Biotin)RFP 适用: Discosoma WB, ELISA, IHC 宿主: 兔 Polyclonal Biotin
anti-Red Fluorescent Protein (RFP) antibody (Biotin)RFP 适用: Discosoma WB, ELISA, IF, FM 宿主: 小鸡 Polyclonal Biotin
anti-Red Fluorescent Protein (RFP) (N-Term) antibodyRFP 适用: Discosoma WB, ELISA, IF, IHC, IP, DB 宿主: 小鼠 Monoclonal RF5R unconjugated
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应用备注
- Application Note: Polyclonal anti-mCherry is designed to detect mCherry, RFP, and its variants. Anti-mCherry (Discosoma sp.) has been tested by ELISA and Western blot and is intended for use in immunological assays including ELISA, western blotting, immunofluorescence, and fluorescence activated cell sorting (FACS). Researchers should determine optimal titers for applications that are not stated. In addition, we performed conjugation of RFP antibodies to either fluorescent dyes, biotin or horseradish peroxidase to further facilitate RFP protein detection and quantification. Immunohistochemistry Dilution: 1:200 - 1:2,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:150,000 - 1:250,000 IF Microscopy Dilution: 1:200 - 1:2,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.04 mg/mL
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缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- RFP (Red Fluorescent Protein (RFP))
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别名
- DsRed
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背景
- Background: mCherry antibody is ideal for western blotting. Fluorescent proteins such as Discosoma Red Fluorescent Protein (and its variants), and GFP are widely used in research practice. Both commonly serve as a markers for gene expression and protein localization. DsRed was isolated from sea anemone Discosoma sp. mushroom and GFP is originated from Aequorea victoria jellyfish. As DsRed and GFP share only 19 % identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional steps in the chromophore maturation and obligates tetrameric structure. All mCherry antibodies have been pre-absorbed to eliminate any potential cross-reactivity to human, mouse and rat serum proteins. The antibodies are also confirmed for non-reactivity to GFP protein.
抗原
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