MST1R 抗体
Quick Overview for MST1R 抗体 (ABIN7565838)
抗原
See all MST1R 抗体适用
宿主
克隆类型
标记
应用范围
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原理
 - RON phospho Y1360 Antibody
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交叉反应 (详细)
 - Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
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产品特性
 - Synonyms: rabbit anti-RON pY1360 antibody, Macrophage-stimulating protein receptor, MSP receptor, Protein-tyrosine kinase 8, p185-Ron, PTK8, RON, c-met-related tyrosine kinase, CD136
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纯化方法
 - Anti-RON pY1360 was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorptions against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
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过滤
 - Sterile filtered
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免疫原
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Immunogen: Anti-RON pY1360 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y1360 of human RON protein.
Immunogen Type: Conjugated Peptide
 
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应用备注
 - Application Note: Anti-RONpY1360 Antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 152.3 kDa corresponding to full length protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human RON pY1360 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Western Blot Dilution: 1:500-1:2000 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
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限制
 - 仅限研究用
 
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状态
 - Liquid
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浓度
 - 0.79 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 
                                            
储存液
 - Sodium azide
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注意事项
 - This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
 - -20 °C
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储存方法
 - Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
 - 12 months
 
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- MST1R (Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R))
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别名
 - MST1R
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背景
 - Background: RON pY1360 antibody detects phosphorylated RON. RON is a member of the protein kinase superfamily. Protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. RON is a heterodimer composed of an α chain and a β chain. The α chain is completely extracellular, whereas the β chain traverses the cell membrane and contains the intracellular tyrosine kinase and regulatory elements. Anti-RON Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.
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基因ID
 - 4486
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NCBI登录号
 - NP_002438
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UniProt
 - Q04912
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途径
 - RTK signaling
 
抗原
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