MEK2 抗体 (C-Term)
Quick Overview for MEK2 抗体 (C-Term) (ABIN7565797)
抗原
See all MEK2 (MAP2K2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- MEK2 C-Term Antibody
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交叉反应 (详细)
- This affinity purified antibody is directed against human MEK2 protein.
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产品特性
- Synonyms: rabbit anti-MEK2 antibody, Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, MAP2K2, MEK, MEK 2, MKK2, PRKMK2, CFC4, MEK-2, ERK activator kinase 2, MAPK/ERK kinase 2
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纯化方法
- Anti-MEK2 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-MEK2 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus human MEK2 protein conjugated to KLH.
Immunogen Type: Conjugated Peptide
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应用备注
- Application Note: Anti-MEK 2 (RABBIT) antibody has been tested in ELISA, Western Blotting, and IHC. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 44 kDa. Immunohistochemistry Dilution: 1:100 Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 5.0 μg/mL
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.09 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))
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别名
- MAP2K2
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背景
- Background: MEK2 antibodies detect the MEK2 isoform. Mitogen-activated protein kinase kinase 2, also known as MEK2 or MKK2, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 2 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. MEK1 and MEK2 are about 80 % identical to each other, and nearly identical within the kinase domain. The MEK2 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.
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UniProt
- P36507
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途径
- MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
抗原
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