ERK1 抗体 (N-Term)
Quick Overview for ERK1 抗体 (N-Term) (ABIN7565757)
抗原
See all ERK1 (MAPK3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- ERK1 N-Term Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human ERK1 protein.
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产品特性
- Synonyms: rabbit anti-ERK-1 Antibody, MAPK3, ERK 1, ERK1, PRKM3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, microtubule-associated protein 2 kinase, p44-ERK1
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纯化方法
- Anti-ERK1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-ERK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus conjugated to KLH.
Immunogen Type: Conjugated Peptide
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anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 101-200) antibody
MAPK3 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, IHC (p), IF (p), IF (cc), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (pTyr204) antibodyMAPK3 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 1-100) antibodyMAPK3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibodyMAPK3 适用: 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibodyMAPK3 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1E5 unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 75-312) antibodyMAPK3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 75-312) antibodyMAPK3 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (N-Term) antibodyVerified MAPK3 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (pThr185), (pThr202), (pTyr187), (pTyr204) antibodyMAPK3 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 279-379) antibodyMAPK3 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 3C9 unconjugated
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应用备注
- Application Note: Anti-ERK 1 (RABBIT) antibody has been tested in ELISA and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43 kDa. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 1:40,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
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别名
- MAPK3
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背景
- Background: ERK1 antibodies detect the ERK1 isoform. Mitogen activated protein kinase 3, also known as MAPK3, ERK, or ERK1, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. ERK1 and ERK2 are activated by MEK1 and MEK2 in the B-raf signaling pathway resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Human ERK1 and ERK2 are 84 % identical in sequence and share common functionality in cells. Anti-ERK1 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.
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UniProt
- P27361
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途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins
抗原
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