Recombinant IL-18 抗体
Quick Overview for Recombinant IL-18 抗体 (ABIN7543421)
抗原
See all IL-18 (IL18) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-IL-18 [ABT-325 (A-794722.0, 2.5(E))], Human IgG1, kappa
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特异性
- This antibody binds human and cynomolgus monkey IL-18 and does not cross react with mouse, rat or dog IL-18. Interleukin-18 is a pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses.
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交叉反应
- Cynomolgus
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产品特性
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Original Species of Ab: Human
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing transgenic Xenomouse with human IL-18 in phosphate-buffered saline.
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亚型
- IgG1 kappa
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anti-Interleukin 18 (IL18) (AA 37-194) antibody
IL18 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 37-193) antibodyIL18 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C14 unconjugated
anti-Interleukin 18 (IL18) (Internal Region) antibodyVerified IL18 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 37-193) antibodyIL18 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 36-192) antibodyIL18 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 94-193) antibodyIL18 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Interleukin 18 (IL18) (AA 32-192) antibody (APC)IL18 适用: 兔 WB, IHC, IP, ICC 宿主: 豚鼠 Polyclonal APC
anti-Interleukin 18 (IL18) (AA 36-192) antibodyIL18 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 37-194) antibodyIL18 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C6 unconjugated
anti-Interleukin 18 (IL18) (AA 32-192) antibodyIL18 适用: 兔 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D3 unconjugated
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应用备注
- The binding characterization of this antibody to human and cynomolgus monkey IL-18 was done using BIACORE assay. The binding affinity of the human IgG1 version was reported to be Kd=0.196 nM. This antibody can be used to immunoprecipitate human and cynomolgus monkey IL-18. This antibody neutralized both recombinant (KG-1 and RBA) and natural IL-18 (WBA) in an in vitro assay. The ability of this antibody to neutralize natural human IL-18-induced IFNγ in an inflammatory environment in vivo, the severe combined immunodeficient (SCID) mouse model was used wherein human PBMCs were injected into the mouse and the cells were stimulated in vivo to produce human IL-18 (HuPBMC-SCID model). This antibody inhibited human IL-18-dependent human IFNγ production in vivo with a clear dose-response by either route of administration. The binding of C1q to this antibody was determined using standard ELISA techniques (US20050100965). This antibody was used in the generation of a dual variable domain immunoglobulin (DVD-Ig) in combination with anti-IL-12 antibody for therapeutic use for targeting disease where multiple mediators contribute to overall disease pathogenesis (PMID: 17934452).
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- IL-18 (IL18) (Interleukin 18 (IL18))
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别名
- IL-18
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背景
- Interleukin-18, IL18, IGIF, IL1F4, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, ABT325, 2.5(E)mg1
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UniProt
- Q14116
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途径
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
抗原
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