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IL-18 抗体 (Internal Region)

Verified IL18 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN570987
发货至: 中国
  • 抗原 See all IL-18 (IL18) 抗体
    IL-18 (IL18) (Interleukin 18 (IL18))
    抗原表位
    • 30
    • 10
    • 7
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    适用
    • 67
    • 37
    • 31
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    宿主
    • 105
    • 24
    • 1
    • 1
    山羊
    克隆类型
    • 102
    • 25
    • 2
    多克隆
    标记
    • 63
    • 16
    • 12
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This IL-18 antibody is un-conjugated
    应用范围
    • 78
    • 46
    • 34
    • 31
    • 26
    • 25
    • 11
    • 11
    • 9
    • 8
    • 4
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    原理
    IL18
    序列
    NPPDNIKDTK SDI
    特异性
    This antibody is expected to recognise both reported isoforms (NP_001553.1, NP_001230140.1).
    交叉反应
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-NPPDNIKDTKSDI, from the internal region of the protein sequence according to NP_001553.1, NP_001230140.1.
    亚型
    IgG
    Top Product
    Discover our top product IL18 Primary Antibody
  • 应用备注

    Western Blot: Approx 23 kDa band observed in lysates of cell line HeLa and approx. 21-22 kDa band in lysates of cell lines A431 and U2OS (calculated MW of 22.3 kDa according to NP_001553.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the golgi and cytoplasm of A431and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conce

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    IL-18 (IL18) (Interleukin 18 (IL18))
    别名
    IL18 (IL18 产品)
    别名
    Igif antibody, Il-18 antibody, IL-18 antibody, IGIF antibody, ChIL-18 antibody, IL-1g antibody, IL1F4 antibody, IL18 antibody, interleukin 18 antibody, Il18 antibody, IL18 antibody
    背景
    IL18, interleukin 18 (interferon-gamma-inducing factor), IGIF, IL-18, IL-1g, IL1F4, MGC12320, IL-1 gamma, interferon-gamma-inducing factor, interleukin 18, interleukin-1 gamma, interleukin-18
    基因ID
    3606, 29197
    NCBI登录号
    NP_001553, NP_001230140
    途径
    Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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