Recombinant IL-8 抗体
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北京 101111
Quick Overview for Recombinant IL-8 抗体 (ABIN7543350)
抗原
See all IL-8 (IL8) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-IL-8 [WS-4], Rabbit IgG, kappa
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特异性
- This antibody binds human IL-8, which is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. This antibody cross reacts with rabbit IL-8.
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交叉反应
- 兔
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产品特性
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Original Species of Ab: Human
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing BALB/c mice with human IL-8. This reshaped WS-4 antibody was generated by grafting CDRs of mouse monoclonal antibody onto human framework regions.
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亚型
- IgG kappa
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anti-Interleukin 8 (IL8) (AA 23-101) antibody
IL8 适用: 马 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) (AA 21-99) antibodyIL8 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) antibodyIL8 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Interleukin 8 (IL8) (AA 72-99) antibodyIL8 适用: 人 WB, IHC, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) antibodyIL8 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) (AA 72-99), (C-Term) antibodyIL8 适用: 人 WB, FACS, IHC (p), IF 宿主: 兔 Polyclonal RB20676 unconjugated
anti-Interleukin 8 (IL8) (AA 23-101) antibodyIL8 适用: 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) (AA 23-101) antibodyIL8 适用: 犬 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Interleukin 8 (IL8) (AA 28-99) antibodyIL8 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) (AA 23-99) antibodyIL8 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- This antibody was used in the generation of a very sensitive ELISA for the detection of human IL-8 in various body fluids. The original mouse version of this antibody has a detection limit of 16 pg/mL in an ELISA. This antibody can bind both the 72 and 77 amino acid forms of IL-8 produced by cultured human cells and cell lines stimulated with IL-1 (PMID: 1593135). The neutralization activity of this antibody towards IL-8 was studied in a rabbit model of severe acute pancreatitis. It was reported that this antibody can reduce the acute lung injury, presumably through inhibition of circulating IL-8 and TNF-alpha, and CD11b/CD18 in lung tissue (PMID: 10189850). This antibody can neutralize rabbit IL-8 in vivo (PMID: 8347559). The effect of neutralizing antibody on IL-8 enhanced infectious cytomegalovirus (CMV) production was examined and it was reported that this antibody reduced virus production nearly to unstimulated levels at 1,000 ng/mL (PMID: 7933146). This antibody was used in the immunohistochemical staining and immuno-localization of IL-8 in tissue sections taken from intestinal loops of patients infected with either M90T or BS176 strains of Shigella flexneri (PMID: 10024597).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- IL-8 (IL8) (Interleukin 8 (IL8))
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别名
- IL-8
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背景
- IL8, Interleukin-8, CXCL8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, GCP-1, Monocyte-derived neutrophil chemotactic factor, MDNCF, Monocyte-derived neutrophil-activating peptide, MONAP, Neutrophil-activating protein 1, NAP-1, Protein 3-10C, T-cell chemotactic factor
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UniProt
- P10145
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途径
- TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy
抗原
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