Recombinant CD40 抗体 (Extracellular Domain)
Quick Overview for Recombinant CD40 抗体 (Extracellular Domain) (ABIN7543234)
抗原
See all CD40 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Extracellular Domain
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原理
- Anti-CD40 [1C10], Rabbit IgG, kappa
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特异性
- This antibody binds mouse CD40, which is a member of the tumor necrosis factor receptor superfamily. CD40 is an integral membrane protein found on the surface of B lymphocytes.
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产品特性
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Original Species of Ab: Rat
Original Format of Ab: IgG2a
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing a rat with 20 mug of soluble extracellular domain of recombinant murine CD40 (sCD40).
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亚型
- IgG kappa
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anti-CD40 (CD40) (AA 26-187) antibody
CD40 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD40 (CD40) antibodyCD40 适用: 人 FACS, IHC, IF, StM, DNA Mic 宿主: 小鼠 Monoclonal C40-1605 unconjugated
anti-CD40 (CD40) (AA 29-107) antibodyCD40 适用: 人 FACS, ELISA, IHC, StM, Coat 宿主: 小鼠 Monoclonal C40-2383 unconjugated
anti-CD40 (CD40) (C-Term) antibodyCD40 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD40 (CD40) (AA 21-193) antibodyCD40 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 4C3B8 unconjugated
anti-CD40 (CD40) (Extracellular Domain) antibodyCD40 适用: 人 FACS, IHC, St 宿主: 小鼠 Monoclonal 5C3 unconjugated
anti-CD40 (CD40) (AA 1-360) antibodyCD40 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD40 (CD40) antibodyCD40 适用: 人 FACS, IHC, IP, Act 宿主: 兔 Chimeric G28-5 unconjugated Recombinant Antibody
anti-CD40 (CD40) antibodyCD40 适用: 人 FACS 宿主: 小鼠 Monoclonal HI40a unconjugated
anti-CD40 (CD40) antibody (FITC)CD40 适用: 人 FACS 宿主: 小鼠 Monoclonal HI40a FITC
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应用备注
- This antibody was reported to directly induce a specific proliferative response from mature murine B cells, up-regulate several B cell surface antigens, and rescue immature B lymphoma cells from anti-IgM-induced growth arrest. This initial screening of hybridoma supernatants for reactivity towards CD40 transfected L cells was done using flow cytometry. This antibody recognized recombinant CD40 expressed on L cells and the soluble extracellular domain of murine CD40 coated onto microtiter plates in an ELISA. This antibody was also used in the immunofluorescent staining of adult murine spleen cells. The staining was restricted to the B220+ population. This antibody also specifically immunoprecipitated a 45- kDa protein from the surface of purified murine splenic B cells (PMID: 7519997). This antibody was capable of blocking binding of FGK45 to CD40 on murine B cells in vitro (PMID: 24416732). The mouse IgG1 version of this antibody had a tenfold higher affinity for mouse FcγRIIB as compared to mouse IgG2a or the parental rat IgG2b (PMID: 21852502). The mouse IgG1 version of this antibody was used in the treatment of M38 tumor bearing mice and it was reported that there was a strong increase in PD-L1 expression both on tumor-infiltrating monocytes and TAMs (PMID: 25623164).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format for improved compatibility with existing reagents assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- CD40
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别名
- CD40
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背景
- TNFRSF5, B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, MGC9013, T-cell differentiation antigen, Tumor necrosis factor receptor superfamily member 5, tumor necrosis factor receptor superfamily, member 5
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UniProt
- P27512
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途径
- NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
抗原
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