Recombinant MUC1 抗体
Quick Overview for Recombinant MUC1 抗体 (ABIN7543226)
抗原
See all MUC1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-MUC1 [12D10], Rabbit IgG, kappa
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特异性
- This antibody is specific for the unsubstituted O-6 position of the Neu5Ac residue of MUC1. The alpha subunit of MUC1 has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit of MUC-1 contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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免疫原
- This antibody was raised by immunizing female Balb/c mice with MUC1 antigen.
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亚型
- IgG kappa
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anti-Mucin 1 (MUC1) (AA 474-630) antibody
MUC1 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 1 (MUC1) (AA 410-525) antibodyMUC1 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 1 (MUC1) antibodyMUC1 适用: 人, 小鼠 IHC, WB, ELISA, FACS, IF, Coat, StM 宿主: 小鼠 Monoclonal MUC1-955 unconjugated
anti-Mucin 1 (MUC1) antibodyMUC1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal MUC1-520 unconjugated
anti-Mucin 1 (MUC1) antibodyMUC1 适用: 人 IHC, WB, IHC (p) 宿主: 小鼠 Monoclonal 2E3 unconjugated
anti-Mucin 1 (MUC1) (AA 1182-1255) antibodyMUC1 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Mucin 1 (MUC1) (AA 66-175) antibodyMUC1 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 3B10B4 unconjugated
anti-Mucin 1 (MUC1) (AA 1098-1255) antibodyMUC1 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 1 (MUC1) (AA 404-655) antibodyMUC1 适用: 大鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 1 (MUC1) (AA 1062-1139) antibodyMUC1 适用: 人, 小鼠, 大鼠 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- To characterize this antibody an ELISA was preformed on MUC1 glycorproteins. Furthermore, the binding constant of this antibody was determined by surface plasmon resonance on MUC1 glycoprotein using the mouse version of this antibody. Finally, human breast cancer cells (T-47D), human lung adenocarcinoma cells (Calu-3), human mucoepidermoid carcinoma cells (H292), and human lung adenocarcinoma epithelial cells were used for flow cytometry with the murine version of this antibody (Naito et al, 2017, pmid:30023556).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- MUC1 (Mucin 1 (MUC1))
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别名
- MUC1
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背景
- Mucin-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin, CD227
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UniProt
- P15941
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途径
- Negative Regulation of intrinsic apoptotic Signaling
抗原
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