MUC1 抗体
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北京 101111
Quick Overview for MUC1 抗体 (ABIN6940099)
抗原
See all MUC1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody
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特异性
- In Western blotting, it recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Human milk-fat globule membranes (HMFGM)
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亚型
- IgG2a, lambda
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应用备注
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Positive Control: MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.
Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- MUC1 (Mucin 1 (MUC1))
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别名
- MUC1
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背景
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Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen, Tumor-associated mucin
Colon, endometrial carcinoma., MCF-7 or MDA-231 cells. Breast, Ovarian, Kidney, Lung
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分子量
- 265-400kDa
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基因ID
- 4582, 89603
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UniProt
- P15941
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途径
- Negative Regulation of intrinsic apoptotic Signaling
抗原
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