Recombinant CEA 抗体
Quick Overview for Recombinant CEA 抗体 (ABIN7543224)
抗原
See all CEA 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-CEA [T84.66], Rabbit IgG, kappa
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特异性
- This antibody binds the A3 domain of the human carcinoembryonic antigen.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing female BALB/c mice subcutaneously with soluble CEA or with the HC84S cell line.
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亚型
- IgG kappa
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anti-Carcinoembryonic Antigen Gene Family (CEA) (AA 448-572) antibody
CEA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) antibodyCEA 适用: 人 WB, ELISA, IHC (p), IF (p) 宿主: 小鼠 Monoclonal B5 unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) (AA 315-365) antibodyCEA 适用: 人 WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) antibodyCEA 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 1C7 unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) antibodyCEA 适用: 人 ELISA, ICC 宿主: 小鼠 Monoclonal 3G12 unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) (AA 400-429) antibodyCEA 适用: 人 WB, IHC, FACS, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) antibodyCEA 适用: 人 ELISA, IHC (p), IF (p), IF (cc) 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) antibodyCEA 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-465R unconjugated Recombinant Antibody
anti-Carcinoembryonic Antigen Gene Family (CEA) (AA 566-698) antibodyCEA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) (AA 544-771) antibodyCEA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- The binding affinity of the original mouse antibody to carcinoembryonic antigen was determined using a competitive radioimmunoassay (PMID: 6187848). 111In (Indacea) labelled version of this antibody was used to image tumors in patients with colorectal cancer (PMID: 3779658). An engineered antibody fragment (VL-VH-CH3, or "minibody") generated using this antibody having bivalent binding to carcinoembryonic antigen showed high level targeting of xenografts (PMID: 8674062). A radiolabeled chimeric version of this antibody was used to target breast cancer cells expressing high levels of CEA and it was found that chimeric 86.66 resulted in good tumor localization as well as significant tumor growth inhibition. This antibody was also used to detect CEA expression in Human breast carcinoma cell line, MCF 7, using flow cytometry and immunohistochemistry (PMID: 21597719). The characterization of the chimeric version of this antibody generated by transient expression in tobacco leaves was done using ELISA (PMID: 10500141). A phase 1 clinical radioimmunotherapy trial studying 22 patients with metastatic CEA-producing malignancies involving 90Yttrium-labeled chimeric T86.44 revealed that the antibody was well tolerated, with reversible thrombocytopenia and leukopenia being dose limiting (PMID: 11051230). This antibody was used in the immunohistochemical staining of paraffin sections of primary tumours from 252 consecutive patients with breast carcinomas (PMID: 9635845).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- CEA (Carcinoembryonic Antigen Gene Family (CEA))
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别名
- CEA
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背景
- Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, CEACAM5, Meconium antigen 100, CD66e
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UniProt
- P06731
抗原
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