Recombinant CD73 抗体
Quick Overview for Recombinant CD73 抗体 (ABIN7543159)
抗原
See all CD73 (NT5E) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-CD73 [AD2], Rabbit IgG, kappa
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特异性
- This antibody is specific for human CD73, which is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase. This antibody does not show cross reactivity with mouse CD73.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing mice with full-length CD73 purified from human placenta.
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亚型
- IgG kappa
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应用备注
- The enzymatic activity of CD73 causes hydrolysis of extracellular nucleotides into membrane permeable nucleosides. This antibody promotes cell-surface clustering of CD73 followed by internalization, thus inhibiting the ability of circulating tumor cells to extravasate and colonize, leading to inhibition of metastasis. This antibody inhibited development of metastasis in a spontaneous animal model of human metastatic breast cancer. Flow cytometry analysis showed that anti-CD73 AD2 mAb (AD2 mAb) recognized CD73 on the human breast cancer cell lines LM3 and MDA-MB-231, but failed to detect the murine CD73 variant expressed on isolated mouse mononuclear hematopoietic cells(PMID: 24043904). This antibody was also used to study the expression of CD73 in normal mammary gland and breast carcinoma cells using immunohistochemistry (PMID: 1989648). mAb AD2 was also used in the immunofluorescence staining for detection of human CD73 in the thymus, peripheral blood, spleen, and lymph node in transgenic mice (PMID: 9045870). Bispecific antibody generated using 1E9 and mAb AD2 is reported to inhibit the enzymatic activity of CD73 as well as promotes internalization of CD73.
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
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别名
- CD73
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背景
- NT5E, 5'-NT, 5'-nucleotidase, Ecto-5'-nucleotidase
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UniProt
- P21589
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途径
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
抗原
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