Recombinant CD25 抗体
Quick Overview for Recombinant CD25 抗体 (ABIN7543079)
抗原
See all CD25 (IL2RA) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-CD25 [7G7/B6], Rabbit IgG, kappa
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特异性
- This antibody is specific for human interleukin 2 (IL2) receptor alpha (IL2RA), which is expressed by activated T cells. IL2R alpha chains can exist as low-affinity monomers, or can non-covalently associate with IL2R beta chains to form high-affinity dimers.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG2a
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纯化方法
- Protein A affinity purified
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免疫原
- This antibody was raised by injecting mice intraperitoneally and subcutaneously with Hong Kong influenza virus-stimulated PBMC, which express human IL-2 receptor alpha. Immune splenocytes were subsequently fused with SP2/0 cells to produce stable hybridomas.
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亚型
- IgG kappa
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anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 201-268) antibody
IL2RA 适用: 人, 小鼠 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibodyIL2RA 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 1B5D12 unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) antibodyIL2RA 适用: 人 WB, ELISA, ICC, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibodyIL2RA 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-245) antibodyIL2RA 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) antibodyIL2RA 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 42-183) antibodyIL2RA 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal IL2RA-2395 unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) antibodyIL2RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-181 unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (PerCP)IL2RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-181 PerCP
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-235) antibodyIL2RA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- This antibody recognises human IL2RA, as confirmed by immunofluorescence, FACS and immunoprecipitation analysis (Rubin et al, 1985). Sequential immunoprecipitations have shown that 7G7/B6 binds to the same 50-55 kD surface molecule as anti-Tac, a murine mAb specific for the human IL-2 receptor. However, 7G7/B6 does not compete with IL-2 and anti-Tac for binding to IL2R expressed by activated PBMCs, indicating that 7G7/B6 recognises a distinct epitope on IL2R. 7G7/B6, therefore, recognises a different epitope on IL-2Rα to the humanised anti-Tac monoclonal antibody daclizumab (Zhang et al, 2006). Equally, although 7G7/B6 binds to a different epitope on IL2 Rα to IL-2, and is, therefore, unable to block IL-2/IL2 Rα interaction, 7G7/B6 is still able to delay tumour progression and prolong survival in mouse models of adult T-cell leukaemia (ATL) (Phillips et al, 2000), likely by FcR-mediated death by FcRIII-expressing cells. Administration of 7B7/B6 radiolabelled with astatine-211 (211At-7G7/B6) to karpas299 leukaemia-bearing mice significantly increased survival, highlighting the potential therapeutic role for 211At-7G7/B6 in the treatment of patients with CD25-expressing leukaemias (Zhang et al, 2006). Indeed, 211At-7G7/B6 in combination with daclizumab induces significant long-term survival in murine models of adult T-cell leukaemia (Zhang et al, 2006). Additionally, 7G7/B6 armed with Yttrium-90 (90Y) significantly extends the survival of SUDHL-1 lymphoma-bearing mice significantly (Zhang et al, 2009), and presents another option for combination therapy with daclizumab. 7G7/B6 has been used in flow cytometric measurements of energy transfer efficiencies between FITC- and Cy3-conjugated mAbs bound to IL-2Rα, β, and γ subunits on Kit 225 K6 T cells, revealing that IL-2R subunits are preassembled on resting cells (Damjanovich et al, 1997). This antibody has also been used in an ELISA to assess serum levels of IL-2R (Uziel et al, 1994).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
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别名
- CD25
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背景
- IL2-RA, Interleukin-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, TAC antigen, p55, Receptor for interleukin-2
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UniProt
- P01589
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途径
- JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
抗原
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