SMAD2 抗体 (pSer467)
Quick Overview for SMAD2 抗体 (pSer467) (ABIN7540643)
抗原
See all SMAD2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer467
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Supplier Product No.
- 600-401-mr3s
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Supplier
- Rockland
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原理
- SMAD2 phospho S467 Antibody
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交叉反应 （详细）
- Anti-SMAD2 pS467 antibody is directed against the phosphorylated form of human Smad2 protein at the pS467 residue.
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
- SMAD2 pS467 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal of human SMAD2 protein.
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亚型
- IgG
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anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibody
SMAD2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3G9 unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 2-467) antibodySMAD2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pSer423), (pSer425), (pSer465), (pSer467) antibodySMAD2 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (C-Term) antibodySMAD2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibodySMAD2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4D10 unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pSer467) antibodySMAD2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pThr183), (pThr221) antibodySMAD2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (N-Term) antibodySMAD2 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibodySMAD2 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2D7 unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 94-112), (N-Term) antibodySMAD2 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA_Dilution: 1.0 μg/mL
Immunohistochemistry_Dilution: 1:2000
IF_Microscopy_Dilution: 15 μg/mL
Western_Blot_Dilution: User Optimized
Other: Dot Blot: 1 μg/mL
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说明
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Anti-SMAD2 pS467 affinity purified antibody has been tested for use in ELISA, DB, IHC, and IF. Expect a band approximately ~49, 52.3 kDa in size corresponding to human Smad2 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Positive controls used: HeLa cells in Immunofluorescence and Human brain cortex in Immunohistochemistry.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
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别名
- SMAD2
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背景
- Rabbit Anti-SMAD2 pS467 antibody, SMAD-2, SMAD 2, mothers against decapentaplegic homolog 2 antibody, MAD homolog 2, Mothers against DPP homolog 2, SMAD family member 2, MADH2, MADH 2, JV18-1,Smad2 (Mothers against decapentaplegic homolog 2) is a member of the Smad family of proteins which are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. Anti-SMAD2 pS467 antibody is ideal for researchers interested in Cancer, Immunology and Nuclear Signaling research.
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基因ID
- 4087
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NCBI登录号
- NP_001003652
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UniProt
- Q15796
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途径
- Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus
抗原
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