p130 抗体 (C-Term)
Quick Overview for p130 抗体 (C-Term) (ABIN7540634)
抗原
See all p130 (RBL2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-mp1
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Supplier
- Rockland
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原理
- p130 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human Retinoblastoma-like protein 2.
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纯化方法
- The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
- Anti-p130 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal portion of human Retinoblastoma-like protein 2 conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG
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anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 939-1139) antibody
RBL2 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2C8A8 unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 165-194), (N-Term) antibodyRBL2 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB31021 unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) antibodyRBL2 适用: 大鼠, 小鼠 IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 100-200) antibodyRBL2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 417-616) antibodyRBL2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) antibodyRBL2 适用: 人, 大鼠, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) antibodyRBL2 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 416-515) antibodyRBL2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 175-189) antibodyRBL2 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 416-515) antibodyRBL2 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4D7 unconjugated
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应用备注
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ELISA_Dilution: 1:10,000 - 1:50,000
Western_Blot_Dilution: 1 μg/mL
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说明
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Anti-p130 Antibody has been tested in ELISA and WB. Expect bands ~128.4 kDa in western blot using appropriate tissues or lysates. Positive control used: MOLT-4 lysate in western blot.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))
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别名
- RBL2
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背景
- Rabbit Anti-p130 Antibody, Rabbit Anti-Retinoblastoma-like protein 2 Antibody, RB Transcriptional Corepressor Like 2, 130 KDa Retinoblastoma-Associated Protein, Retinoblastoma-Related Protein 2, Retinoblastoma-Like Protein 2, Retinoblastoma-Like 2, RBR-2,Retinoblastoma-Associated Protein (130 KDa) is a key regulator of entry into cell division. p130 is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression and controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters and is a potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. It binds to cyclins A and E, as well as, binds to and may be involved in the transforming capacity of the adenovirus E1A protein. p130 may act as a tumor suppressor. Anti-p130 Antibody may be useful for researchers interested in Adipogenesis, Retinoblastoma, and Retinal Cancer.
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基因ID
- 5934
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NCBI登录号
- NP_001310537
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UniProt
- Q08999
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases
抗原
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