Caspase 12 抗体 (Internal Region)
Quick Overview for Caspase 12 抗体 (Internal Region) (ABIN7540612)
抗原
See all Caspase 12 (CASP12) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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Supplier Product No.
- 600-401-mj3s
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Supplier
- Rockland
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原理
- Caspase 12 Antibody
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交叉反应 (详细)
- This affinity purified antibody is directed against mouse Caspase 12.
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纯化方法
- The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
- Anti-Caspase 12 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Caspase 12 conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG
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应用备注
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ELISA_Dilution: 1:10,000 - 1:50,000
IF_Microscopy_Dilution: 15 μg/mL
Western_Blot_Dilution: 5 μg/mL
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说明
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Anti-Caspase 12 Antibody has been tested in ELISA, IF, and WB. Positive control used: NIH/3T3 and PC-12 lysates in Western Blot and NIH/3T3 in Immunofluorescence.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))
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别名
- Casp12
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背景
- Rabbit Anti-Caspase 12 Antibody, Caspase 12 pseudogene 1, Casp12, Casp-12, CASP12P1,Casp12 (Caspase 12 (Gene/Pseudogene)) has no protease activity. It may reduce cytokine release in response to bacterial lipopolysaccharide during infections. Caspase 12 reduces activation of NF-kappa-B in response to TNF. Caspases (cysteinyl aspartate proteases) are involved in the signaling pathways of apoptosis, necrosis and inflammation. These enzymes can be divided into initiators and effectors. The initiator isoforms are activated by, and interact with, upstream adaptor molecules. Anti-Caspase 12 Antibody is useful for researchers interested in Amyotrophic lateral sclerosis (ALS), ATP synthesis, and Apoptosis research.
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基因ID
- 12364
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NCBI登录号
- NP_033938
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UniProt
- O08736
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途径
- Apoptosis, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
抗原
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