PINK1 抗体 (C-Term)
(1 reference)
Quick Overview for PINK1 抗体 (C-Term) (ABIN7540590)
抗原
See all PINK1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-gu5s
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Supplier
- Rockland
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原理
- PINK1 truncated Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human PINK1.
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纯化方法
- The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
- Anti-PINK1 truncated antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Term portion of human PINK1 conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG
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anti-PTEN Induced Putative Kinase 1 (PINK1) (Internal Region) antibody
Verified PINK1 适用: 人 WB, ELISA, FACS 宿主: 山羊 Polyclonal unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-273) antibodyPINK1 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 3A9F8 unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) antibodyPINK1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 小鼠 Monoclonal 38CT20-8-5 unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-273) antibodyPINK1 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5F2B11 unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibodyPINK1 适用: 人 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S4-15 unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (HRP)PINK1 适用: 人 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S4-15 HRP
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (FITC)PINK1 适用: 人 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S4-15 FITC
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (Biotin)PINK1 适用: 人 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S4-15 Biotin
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (PE)PINK1 适用: 人 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S4-15 PE
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (PerCP)PINK1 适用: 人 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S4-15 PerCP
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应用备注
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Immunoprecipitation_Dilution: User Optimized
ELISA_Dilution: User Optimized
Immunohistochemistry_Dilution: 1:200
Flow_Cytometry_Dilution: User Optimized
IF_Microscopy_Dilution: 20 μg/mL
Western_Blot_Dilution: 1 μg/mL
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说明
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Suggested Applications: FC
Anti-PINK1 truncated Antibody has been tested in Western Blot, Immunofluorescence, and ELISA. Positive controls were recombinant PINK1 protein and overexpressed PINK1 transfected HEK293 @ 1μg/mL in western blot. Expect a lower strength band at 62.7 kDa in western blot using appropriate lysates. PINK1 truncated antibody strongly detects endogenously truncated PINK1 protein, seen at ~30kDa. PINK1 is highly phosphorylated and degrades rapidly which may cause additional banding in western blot. Positive control cell lines for immunofluorescence assays are NIH3T3 (p/n W10-000-358). Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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: "CD38 reduces mitochondrial fitness and cytotoxic T cell response against viral infection in lupus patients by suppressing mitophagy." in: Science advances, Vol. 8, Issue 24, pp. eabo4271, (2022) (PubMed).
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: "CD38 reduces mitochondrial fitness and cytotoxic T cell response against viral infection in lupus patients by suppressing mitophagy." in: Science advances, Vol. 8, Issue 24, pp. eabo4271, (2022) (PubMed).
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- PINK1 (PTEN Induced Putative Kinase 1 (PINK1))
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别名
- PINK1
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背景
- Rabbit anti-PINK1 antibody, Serine/threonine-protein kinase PINK1 (mitochondrial), BRPK, PTEN-induced putative kinase protein 1, PTEN-induced kinase,PINK1 (PTEN induced putative kinase 1) belongs to the protein kinase superfamily and Ser/Thr protein kinase family. PINK1 protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. It is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2. Targets PARK2 to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PARK2 in 2 steps: (1) by mediating phosphorylation at Ser-65 of PARK2 and (2) mediating phosphorylation of ubiquitin, converting PARK2 to its fully-active form. PINK1 interacts with PARK2 and FBXO7 and forms a complexes with PARK2 and PARK7. PINK1 is associated with Parkinson disease 6 (PARK6). Anti-PINK1 Antibody is useful for researcher interested in neurological research, neurodegenerative disorders, Pink/Parkin Mediated Mitophagy, and Parkinsons Disease Pathway.
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基因ID
- 65018
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NCBI登录号
- NP_115785
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UniProt
- Q9BXM7
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途径
- Autophagy
抗原
-
