ASCL1 抗体 (Internal Region)
Quick Overview for ASCL1 抗体 (Internal Region) (ABIN7540560)
抗原
See all ASCL1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- Internal Region
-
Supplier Product No.
- 600-101-mh4s
-
Supplier
- Rockland
-
原理
- Mash1/Ascl1 Antibody
-
交叉反应 （详细）
- This affinity purified antibody is directed against mouse Mash1/Ascl1.
-
纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
-
过滤
- Sterile filtered
-
免疫原
- Anti-Mash1/Ascl1 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Mash1/Ascl1 conjugated to Keyhole Limpet Hemocyanin (KLH).
-
亚型
- IgG
-
-
anti-Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1) (AA 151-236) antibody
ASCL1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1) (AA 190-236) antibodyASCL1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1) (AA 137-236) antibodyASCL1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3D3 unconjugated
anti-Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1) (N-Term) antibodyASCL1 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 犬, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1) (AA 84-236) antibodyASCL1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1) (AA 1-236) antibodyASCL1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1) (AA 137-236) antibodyASCL1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1) (AA 137-236) antibodyASCL1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2D9 unconjugated
anti-Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1) (C-Term) antibodyASCL1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1) (AA 48-97) antibodyASCL1 适用: 人, Cow, 豚鼠, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
ELISA_Dilution: 1:10,000-1:50,000
Immunohistochemistry_Dilution: 1:1000
Western_Blot_Dilution: 1:1000
-
说明
-
Anti-Mash1/Ascl1 Antibody has been tested in Western Blot and Immunohistochemistry. Expect a band at ~24.7 kDa in western blot using appropriate lysates or tissues. Positive control used was mouse pancreas in WB, human thyroid papillary carcinoma tissue in IHC.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
-
有效期
- 12 months
-
-
- ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))
-
别名
- Ascl1
-
背景
- Goat Anti-Ascl1 Antibody, Goat Anti-Mash1 Antibody, Achaete-scute homolog 1, ASH-1, mASH-1,Ascl1 (Achaete-scute homolog 1) is a transcription factor that plays a key role in neuronal differentiation, it acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. It directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Ascl1 plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. It is essential for the generation of olfactory and autonomic neurons. It acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Anti-Mash1/Ascl1 Antibody is useful for researchers interested in CCHS, SIDS, Dopaminergic Neurogenesis, and DNA binding.
-
基因ID
- 17172
-
NCBI登录号
- NP_032579
-
UniProt
- Q02067
-
途径
- Dopaminergic Neurogenesis
抗原
-
