His 标记 抗体
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北京 101111
Quick Overview for His 标记 抗体 (ABIN7539828)
抗原
See all His Tag 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- His Tag Antibody
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纯化方法
- Purified antibody
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免疫原
- Synthesized peptide of His Tag (AA: HHHHHHHH-KLH).
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亚型
- IgG1
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anti-His Tag antibody
WB, ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibody适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated
anti-His Tag antibodyWB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated
anti-His Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated
anti-His Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated
anti-His Tag antibody (DyLight 649)WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 649
anti-His Tag antibody (HRP)WB, ELISA, IHC 宿主: 小鼠 Monoclonal 33D10-D2-G8 HRP
anti-His Tag antibody (Cy3)WB, ELISA, FACS, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 Cy3
anti-His Tag antibody (DyLight 800)WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 800
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应用备注
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ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/100 - 1/1000
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限制
- 仅限研究用
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缓冲液
- Purified antibody in PBS with 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
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- His Tag
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物质类
- Tag
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背景
- The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.
抗原
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