Recombinant MUC1 抗体
Quick Overview for Recombinant MUC1 抗体 (ABIN7487742)
抗原
See all MUC1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-MUC1 [HMFG1 (1.10.F3)], Rabbit IgG, kappa
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特异性
- This antibody binds the MUC1, a tumor associated antigen expressed on many breast cancer cells. This antibody does not show reactivity towards fibroblasts, lymphoblastoid cells, and a large number of epithelial cell lines of non-breast origin. This antibody recognizes the 'PDTR' epitope of mucin.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing mice with human mammary milk fat globule.
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亚型
- IgG kappa
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anti-Mucin 1 (MUC1) (AA 474-630) antibody
MUC1 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 1 (MUC1) (AA 410-525) antibodyMUC1 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 1 (MUC1) antibodyMUC1 适用: 人, 小鼠 IHC, WB, ELISA, FACS, IF, Coat, StM 宿主: 小鼠 Monoclonal MUC1-955 unconjugated
anti-Mucin 1 (MUC1) antibodyMUC1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal MUC1-520 unconjugated
anti-Mucin 1 (MUC1) antibodyMUC1 适用: 人 IHC, WB, IHC (p) 宿主: 小鼠 Monoclonal 2E3 unconjugated
anti-Mucin 1 (MUC1) (AA 1182-1255) antibodyMUC1 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Mucin 1 (MUC1) (AA 66-175) antibodyMUC1 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 3B10B4 unconjugated
anti-Mucin 1 (MUC1) (AA 1098-1255) antibodyMUC1 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 1 (MUC1) (AA 404-655) antibodyMUC1 适用: 大鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 1 (MUC1) (AA 1062-1139) antibodyMUC1 适用: 人, 小鼠, 大鼠 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- This antibody reacts with most of the breast cancer cells lines and with epithelial cells of human milk. The antibody also reacts with neoplasms of epithelial origin, particularly carcinomas of breast, ovary, cervix, bronchus and gastrointestinal tract. This antibody was used for the study of expression of epithelial antigen in normal and disordered cervical squamous epithelium using immunohistological methods (PMID: 6315048). This antibody can recognize the epithelial membrane antigen in a radioimmunoassay. Immunofluorescent staining of cultures of human breast cells with these antibodies demonstrated that they are heterogeneous in their distribution (PMID: 6086018). HMFG1 and HMFG2 recognize different epitopes of the millk fat globule (PMID: 6086018, 2424847). This antibody was used for the study of tumor associated antigenic epitopes purified from human skimmed milk using immunoaffinity chromatography, immunoprecipitation and immunoblot techniques (PMID: 2443241). The reshaped humanized antibody, HuHMFGl, showed no difference in relative affinity for its antigen compared with the parent mouse HMFG1 (PMID: 7682986). This mAb reacts with P1-24 synthetic peptide (PDTRPAPGSTAPPAHGVTSAPDTR) representing the repeating amino acid sequence of the mucin core protein and also reacted strongly with ovarian carcinoma-associated sebaceous gland antigen (SGA) by direct binding and in a sandwich ELISA assay with OM-1 as the capture Mab (PMID: 1697426).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- MUC1 (Mucin 1 (MUC1))
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别名
- MUC1
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背景
- CD227, Muc-1, Mucin-1, Milk mucin, human milk fat globule antigen, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA
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UniProt
- P15941
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途径
- Negative Regulation of intrinsic apoptotic Signaling
抗原
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