Recombinant CD40 抗体
Quick Overview for Recombinant CD40 抗体 (ABIN7487699)
抗原
See all CD40 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Anti-CD40 [FGK45], Rabbit IgG, kappa
-
特异性
- This antibody recognizes CD40. It is a type I transmembrane glycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD40 is expressed broadly on antigen-presenting cells (APCs) such as dendritic cells, B cells, macrophages, and monocytes as well as non-immune endothelial cells, basal epithelial cells, and a range of tumors. Upon binding to its ligand CD154, CD40 acts as a costimulatory molecule for the activation of B cells, dendritic cells, monocytes, and other APCs.
-
产品特性
-
Original Species of Ab: Rat
Original Format of Ab: IgG2a
-
纯化方法
- Protein A affinity purified
-
免疫原
- The original rat IgG2a antibody was raised against the recombinant mouse CD40 fusion protein.
-
亚型
- IgG kappa
-
-
anti-CD40 (CD40) (AA 26-187) antibody
CD40 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD40 (CD40) antibodyCD40 适用: 人 FACS, IHC, IF, StM, DNA Mic 宿主: 小鼠 Monoclonal C40-1605 unconjugated
anti-CD40 (CD40) (AA 29-107) antibodyCD40 适用: 人 FACS, ELISA, IHC, StM, Coat 宿主: 小鼠 Monoclonal C40-2383 unconjugated
anti-CD40 (CD40) (C-Term) antibodyCD40 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD40 (CD40) (AA 21-193) antibodyCD40 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 4C3B8 unconjugated
anti-CD40 (CD40) (Extracellular Domain) antibodyCD40 适用: 人 FACS, IHC, St 宿主: 小鼠 Monoclonal 5C3 unconjugated
anti-CD40 (CD40) (AA 1-360) antibodyCD40 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD40 (CD40) antibodyCD40 适用: 人 FACS, IHC, IP, Act 宿主: 兔 Chimeric G28-5 unconjugated Recombinant Antibody
anti-CD40 (CD40) antibodyCD40 适用: 人 FACS 宿主: 小鼠 Monoclonal HI40a unconjugated
anti-CD40 (CD40) antibodyCD40 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 1C10 unconjugated
-
-
应用备注
- FGK45 is an agonistic antibody that has been shown to activate CD40 expressing antigen presenting cells (APCs) what might promote anti-tumor T cell responses. For instance, the rat IgG2a version of this antibody was used to promote maturation of APCs what improved the ability of the macrophages to fight a tumour (Luheshi et al., 2016, pmid: 26918344). Another group used the same antibody to stimulate T-cells in a study on a tumor vaccine (Bartkowiak et al., 2015, pmid: 26351680). Others used ths clone as well to induce potent anti-tumor activity (Carmi et al., 2015, pmid: 25924063). On the other hand, it can also be used to inhibit CD40/CD154 interaction in vitro and in vivo. Furthermore, this clone can be also utilized to identify CD40-positive cells by flow cytometry (Rolink et al., 1996, pmid: 8885865). Administering this antibody can be also utilized to induce inflamatory bowel disease-like characteristics and create a mouse IBD model to study this class of diseases (Bauche et al., 2018, pmid: 30097293).
-
说明
-
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
-
限制
- 仅限研究用
-
-
-
浓度
- 1 mg/mL
-
缓冲液
- PBS with 0.02 % Proclin 300.
-
储存液
- ProClin
-
注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
-
-
- CD40
-
别名
- CD40
-
背景
- Tnfrsf5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, FGK4.5
-
UniProt
- P27512
-
途径
- NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
抗原
-
