UPK1A 抗体 (AA 114-173)
Quick Overview for UPK1A 抗体 (AA 114-173) (ABIN7477611)
抗原
See all UPK1A 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 114-173
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免疫原
- Recombinant fragment (around aa 114-173) of human Uroplakin 1A (UPK1A) protein (exact sequence is proprietary)
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亚型
- IgG
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anti-Uroplakin 1a (UPK1A) (AA 114-173) antibody
UPK1A 适用: 人 ELISA, Coat, IHC (f) 宿主: 小鼠 Monoclonal UPK1A-2921 unconjugated
anti-Uroplakin 1a (UPK1A) (AA 114-173) antibodyUPK1A 适用: 人 ELISA, Coat, IHC (f) 宿主: 小鼠 Monoclonal UPK1A-2925 unconjugated
anti-Uroplakin 1a (UPK1A) antibodyUPK1A 适用: 小鼠, 大鼠 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Uroplakin 1a (UPK1A) (AA 117-232) antibodyUPK1A 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Uroplakin 1a (UPK1A) (AA 116-231) antibodyUPK1A 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Uroplakin 1a (UPK1A) (AA 197-227), (C-Term) antibodyUPK1A 适用: 人 WB, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Urinary bladder: A strong membranous and cytoplasmic Upk1a immunostaining should be seen in the urothelium (the staining can be limited to the top 1/3 or 2/3 of the urothelium).
Negative Control: Colon: Upk1a immunostaining should be absent in all cells of the colon mucosa.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- UPK1A (Uroplakin 1a (UPK1A))
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别名
- Uroplakin 1A
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背景
- UP1A, UPIA, UPKA, TSPAN21,Uroplakin 1A antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- O00322
抗原
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