Recombinant Topoisomerase II alpha 抗体 (AA 1352-1493)
Quick Overview for Recombinant Topoisomerase II alpha 抗体 (AA 1352-1493) (ABIN7477603)
抗原
See all Topoisomerase II alpha (TOP2A) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1352-1493
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原理
- Topoisomerase 2-alpha / TOPO2A antibody (802R)
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免疫原
- Recombinant human Topoisomerase II alpha fragment (aa1352-1493) (exact sequence is proprietary)
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Colon: A fraction of cells, especially in the crypt basis should show a moderate to strong nuclear TOPO2A immunostaining.
Product Description/Background: Topoisomerase 2-alpha / TOPO2A antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Colon: Epithelial cells must not show any TOPO2A staining in the cytoplasm and in most nuclei of the surface epithelium.
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实验流程
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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有效期
- 24 months
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- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
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别名
- Topoisomerase 2-alpha
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背景
- Gene Alias: ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase II 170kD, DNA topoisomerase II alpha, Topoisomerase DNA II alpha 170 kDa, TP2A
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UniProt
- P11388
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases
抗原
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