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Recombinant Topoisomerase II alpha 抗体 (AA 1352-1493)

This anti-Topoisomerase II alpha antibody is a 兔 单克隆 antibody detecting Topoisomerase II alpha in IHC. Suitable for 人.
产品编号 ABIN7477603
发货至: 中国

Quick Overview for Recombinant Topoisomerase II alpha 抗体 (AA 1352-1493) (ABIN7477603)

抗原

See all Topoisomerase II alpha (TOP2A) 抗体
Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This Topoisomerase II alpha antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)

克隆位点

802R
  • 抗原表位

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    AA 1352-1493

    原理

    Topoisomerase 2-alpha / TOPO2A antibody (802R)

    免疫原

    Recombinant human Topoisomerase II alpha fragment (aa1352-1493) (exact sequence is proprietary)

    亚型

    IgG
  • 应用备注

    IHC 1:100-1:200

    说明

    Positive Control: Colon: A fraction of cells, especially in the crypt basis should show a moderate to strong nuclear TOPO2A immunostaining.

    Product Description/Background: Topoisomerase 2-alpha / TOPO2A antibody validated for immunohistochemistry on 76 different Normal Tissues

    Negative Control: Colon: Epithelial cells must not show any TOPO2A staining in the cytoplasm and in most nuclei of the surface epithelium.

    实验流程

    Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

    限制

    仅限研究用
  • 状态

    Liquid

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

    有效期

    24 months
  • 抗原

    Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

    别名

    Topoisomerase 2-alpha

    背景

    Gene Alias: ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase II 170kD, DNA topoisomerase II alpha, Topoisomerase DNA II alpha 170 kDa, TP2A

    UniProt

    P11388

    途径

    Cell Division Cycle, Mitotic G1-G1/S Phases
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