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Recombinant Prostate Specific Antigen 抗体 (AA 150-250)

This anti-Prostate Specific Antigen antibody is a 兔 单克隆 antibody detecting Prostate Specific Antigen in IHC. Suitable for 人.
产品编号 ABIN7477569
发货至: 中国

Quick Overview for Recombinant Prostate Specific Antigen 抗体 (AA 150-250) (ABIN7477569)

抗原

See all Prostate Specific Antigen (PSA) 抗体
Prostate Specific Antigen (PSA)

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This Prostate Specific Antigen antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)

克隆位点

MSVA-603R
  • 抗原表位

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    AA 150-250

    免疫原

    Recombinant fragment (around aa150-250) of human KLK3 protein (exact sequence is proprietary)

    亚型

    IgG
  • 应用备注

    IHC 1:100-1:200

    说明

    Positive Control: Prostate: The epithelial cells of the prostate glands must show a strong cytoplasmic staining. Due to leakage of the antigen in the vicinity of the prostate glands, adjacent stroma cells may display a weak to moderate staining reaction.

    Negative Control: Colon: PSA immunostaining should be absent in all cells.

    实验流程

    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

    限制

    仅限研究用
  • 状态

    Liquid

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • 抗原

    Prostate Specific Antigen (PSA)

    别名

    PSA

    背景

    Antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin,PSA antibody validated for immunohistochemistry on 76 different Normal Tissues

    UniProt

    P07288

    途径

    Complement System
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