Recombinant Prostate Specific Antigen 抗体 (AA 150-250)
Quick Overview for Recombinant Prostate Specific Antigen 抗体 (AA 150-250) (ABIN7477569)
抗原
See all Prostate Specific Antigen (PSA) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 150-250
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免疫原
- Recombinant fragment (around aa150-250) of human KLK3 protein (exact sequence is proprietary)
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Prostate: The epithelial cells of the prostate glands must show a strong cytoplasmic staining. Due to leakage of the antigen in the vicinity of the prostate glands, adjacent stroma cells may display a weak to moderate staining reaction.
Negative Control: Colon: PSA immunostaining should be absent in all cells.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Prostate Specific Antigen (PSA)
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别名
- PSA
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背景
- Antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin,PSA antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P07288
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途径
- Complement System
抗原
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