Recombinant MUC1 抗体
Quick Overview for Recombinant MUC1 抗体 (ABIN7477525)
抗原
See all MUC1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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免疫原
- Recombinant human MUC1 protein
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亚型
- IgG
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应用备注
- IHC 1:75-1:150
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说明
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Positive Control: Kidney: Distal tubuli and collecting ducts must show a strong cytoplasmic MUC1 staining while the staining should be at least moderate in the parietal and visceral sheets of the Bowman's capsule and largely absent in proximal tubuli.
Negative Control: Colon: MUC1 staining should be very weak or absent in colonic epithelium.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- MUC1 (Mucin 1 (MUC1))
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别名
- MUC1 / EMA
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背景
- Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma- associated mucin Episialin, Epithelial Membrane Antigen (EMA), H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1- alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen,MUC1 / EMA antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P15941
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途径
- Negative Regulation of intrinsic apoptotic Signaling
抗原
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