Recombinant CTLA4 抗体
Quick Overview for Recombinant CTLA4 抗体 (ABIN7477429)
抗原
See all CTLA4 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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亚型
- IgG
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应用备注
- IHC 1:50-1:100
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说明
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Positive Control: Tonsil: A moderate strong membranous and cytoplasmic CTLA4 immunostaining should be seen in a subset of T-lymphocytes with strongest staining in follicular T-helper cells of germinal centres.
Negative Control: Tonsil: The majority of lymphocytes in germinal centres and all epithelial cells should stain negative.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
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别名
- CTLA-4
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背景
- ALPS5, CD152, Celiac disease 3 (CELIAC3), Cytotoxic T-lymphocyte-associated antigen 4 (CTLA4), GRD4, GSE, ICOS, Insulin-dependent Diabetes Mellitus 12 (IDDM12),CTLA-4 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P16410
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途径
- Cancer Immune Checkpoints
抗原
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