CD31 抗体 (AA 625-738)
Quick Overview for CD31 抗体 (AA 625-738) (ABIN7477409)
抗原
See all CD31 (PECAM1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 625-738
-
免疫原
- Recombinant fragment (around aa 625-738) of human CD31 protein (exact sequence is proprietary)
-
亚型
- IgG
-
-
-
-
应用备注
- IHC 1:100-1:200
-
说明
-
Positive Control: Liver: At least a weak to moderate staining of the majority of the hepatic sinusoidal endothelial cells should be seen.
Negative Control: Liver: Hepatocytes should not show any CD31 staining.
-
实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
-
- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
-
别名
- CD31
-
背景
- EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA,CD31 antibody validated for immunohistochemistry on 76 different Normal Tissues
-
UniProt
- P16284
-
途径
- Regulation of Actin Filament Polymerization
抗原
-