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CD31 抗体 (AA 625-738)

This anti-CD31 antibody is a 小鼠 单克隆 antibody detecting CD31 in IHC. Suitable for 人.
产品编号 ABIN7477409
发货至: 中国

Quick Overview for CD31 抗体 (AA 625-738) (ABIN7477409)

抗原

See all CD31 (PECAM1) 抗体
CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD31 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)

克隆位点

MSVA-031M
  • 抗原表位

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    AA 625-738

    免疫原

    Recombinant fragment (around aa 625-738) of human CD31 protein (exact sequence is proprietary)

    亚型

    IgG
  • 应用备注

    IHC 1:100-1:200

    说明

    Positive Control: Liver: At least a weak to moderate staining of the majority of the hepatic sinusoidal endothelial cells should be seen.

    Negative Control: Liver: Hepatocytes should not show any CD31 staining.

    实验流程

    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

    限制

    仅限研究用
  • 状态

    Liquid

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • 抗原

    CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

    别名

    CD31

    背景

    EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA,CD31 antibody validated for immunohistochemistry on 76 different Normal Tissues

    UniProt

    P16284

    途径

    Regulation of Actin Filament Polymerization
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