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CD73 抗体

This anti-CD73 antibody is a 兔 多克隆 antibody detecting CD73 in WB, IF, ICC, IHC (p), Inh, Neut 和 PLA. Suitable for 人.
产品编号 ABIN7464558
发货至: 中国

Quick Overview for CD73 抗体 (ABIN7464558)

抗原

See all CD73 (NT5E) 抗体
CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

适用

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克隆类型

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多克隆

标记

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This CD73 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Inhibition (Inh), Neutralization (Neut), Proximity Ligation Assay (PLA)
  • 交叉反应

    人, 小鼠

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human CD73. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: HepG2 , HCT116 , mouse placenta

    Validation: Orthogonal

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.28 mg/mL

    缓冲液

    1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

    别名

    5'-nucleotidase ecto

    背景

    5'-nucleotidase ecto , CALJA , CD73 , E5NT , NT , NT5 , NTE , eN , eNT,Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase, EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

    分子量

    63 kDa

    基因ID

    4907

    UniProt

    P21589

    途径

    Synaptic Membrane, Ribonucleoside Biosynthetic Process
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